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This compendium describes quantitative imaging-based methods designed to provide objective evaluation of several variables that contribute to experimental results.
- Publication Part Number: 5994-3007EN
- Created: 01 Feb 2021
- 1 MB
This application note demonstrates the ability of the Cytation 5 in conjunction with Gen5 analysis to calculate HIV infection rates in fixed cells.
- Publication Part Number: 5994-2542EN
- Created: 01 Feb 2021
- 249 KB
This application note demonstrates the ability to monitor cell migration and invasion using a novel cell imaging multimode reader.
- Publication Part Number: 5994-2751EN
- Created: 01 Feb 2021
- 598 KB
This application note illustrates how the Cytation 3 can be used to image cells that have been cultured into 3D spheroids.
- Publication Part Number: 5994-2520EN
- Created: 01 Feb 2021
- 457 KB
Agilent BioTek microplate readers and Take3 microvolume plate enable rapid indication of protein contamination in nucleic acid isolates using the A260/A280 ratio.
- Publication Part Number: 5994-2538EN
- Created: 01 Feb 2021
- 279 KB
This application note demonstrates a multiplexed assay for inhibitors at the level of EGFR and NFkB to monitor IL-6 secretion and cell viability.
- Publication Part Number: 5994-2552EN
- Created: 01 Feb 2021
- 815 KB
This application note describes the use of the peristaltic pump‑based Agilent BioTek MicroFlo Select to dispense cells into 1,536-well microplates.
- Publication Part Number: 5994-2615EN
- Created: 01 Feb 2021
- 1 MB
Three-dimensional (3D) in vitro cell culture platforms are motivated by the need to work with cellular models that better recapitulate the cellular microenvironment.
- Publication Part Number: 5994-2571EN
- Created: 01 Feb 2021
- 1 MB
| Korean (Korea) | Complete (PDF) |
|---|---|
| Chinese (Simplified) (China) | Complete (PDF) |
This application note demonstrates the optimization and validation of cellular energy flux probes using a variety of Agilent BioTek readers and detection methods.
- Publication Part Number: 5994-2586EN
- Created: 01 Feb 2021
- 617 KB
This application note demonstrates an in vitro live cell assay to monitor NETosis activity using differentiated HL-60 cells as a model.
- Publication Part Number: 5994-2572EN
- Created: 01 Feb 2021
- 3 MB
Several colorimetric and fluorescent assays are described incorporating automated image acquisition and analyses using Agilent’s high-content imaging instruments.
- Publication Part Number: 5994-3068EN
- Created: 01 Feb 2021
- 9 MB
| Chinese (Simplified) (China) | Complete (PDF) |
|---|---|
| Korean (Korea) | Complete (PDF) |
This application note demonstrates that vascular growth is repressed by VEGF inhibitors in a dose‑dependent manner.
- Publication Part Number: 5994-2752EN
- Created: 01 Feb 2021
- 323 KB
The alkaline comet assay is a robust method to perform genotoxicity measurements and, with the addition of the 96-well CometSlide and CometChip, analysis is efficiently performed on large sample numbers in a high-throughput format.
- Publication Part Number: 5994-2597EN
- Created: 01 Feb 2021
- 496 KB
This application note describes the use of the EL406 in conjunction with BioStack and LHC software to semi-automate a melamine ELISA.
- Publication Part Number: 5994-2689EN
- Created: 01 Feb 2021
- 165 KB
This study describes an effective method for automating the most tedious aspects of conducting ELISpot assays using the versatile 405 TS microplate washer.
- Publication Part Number: 5994-3025EN
- Created: 01 Feb 2021
- 593 KB
This study demonstrates the utility of a 3D spheroid cell culture model, used to elucidate the apoptotic potential of two compounds in two different cell lines.
- Publication Part Number: 5994-2591EN
- Created: 01 Feb 2021
- 471 KB
This study describes a method for detecting Escherichia coli O157:H7, Listeria monocytogen, and Salmonella enterica Typhimurium in spiked lettuce wash samples.
- Publication Part Number: 5994-2547EN
- Created: 01 Feb 2021
- 547 KB
This compendium describes how fluorescent kinetic live cell assay procedures can be optimized and performed using cellular imaging and automation instrumentation.
- Publication Part Number: 5994-2567EN
- Created: 01 Feb 2021
- 2 MB
| Chinese (Simplified) (China) | Complete (PDF) |
|---|---|
| Korean (Korea) | Complete (PDF) |
This application note shows the use of a filter-based absorbance microplate reader for the enzymatic analysis of a member of the dehydrogenase family of proteins.
- Publication Part Number: 5994-2532EN
- Created: 01 Feb 2021
- 261 KB
This application note evaluates the ability to detect changes in ERK/CREB phosphorylation levels post treatment with Aβ using the SH-SY5Y neuroblastoma cell line.
- Publication Part Number: 5994-2527EN
- Created: 01 Feb 2021
- 2 MB