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Several colorimetric and fluorescent assays are described incorporating automated image acquisition and analyses using Agilent’s high-content imaging instruments.
- Publication Part Number: 5994-3068EN
- Created: 01 Feb 2021
- 9 MB
| Chinese (Simplified) (China) | Complete (PDF) |
|---|---|
| Korean (Korea) | Complete (PDF) |
This study describes a method to incorporate automated, digital widefield phase contrast microscopy to monitor potential apoptotic effects of lead molecules.
- Publication Part Number: 5994-2589EN
- Created: 01 Feb 2021
- 840 KB
The Cytation 5, paired with BioSpa automated incubator, provides a fully automated means of quantifying image-based assays of cell proliferation and cell death.
- Publication Part Number: 5994-2593EN
- Created: 01 Feb 2021
- 644 KB
This application note demonstrates an in vitro live cell assay to monitor NETosis activity using differentiated HL-60 cells as a model.
- Publication Part Number: 5994-2572EN
- Created: 01 Feb 2021
- 3 MB
This application note evaluates chemically induced cell stress by thapsigargin, while simultaneously measuring its effects on Gq-mediated cell signaling.
- Publication Part Number: 5994-2587EN
- Created: 01 Feb 2021
- 758 KB
This application note demonstrates that vascular growth is repressed by VEGF inhibitors in a dose‑dependent manner.
- Publication Part Number: 5994-2752EN
- Created: 01 Feb 2021
- 323 KB
The alkaline comet assay is a robust method to perform genotoxicity measurements and, with the addition of the 96-well CometSlide and CometChip, analysis is efficiently performed on large sample numbers in a high-throughput format.
- Publication Part Number: 5994-2597EN
- Created: 01 Feb 2021
- 496 KB
This application compendium covers brightfield and fluorescent microscopy methods to monitor cell proliferation and viability.
- Publication Part Number: 5994-2568EN
- Created: 01 Feb 2021
- 3 MB
| Chinese (Simplified) (China) | Complete (PDF) |
|---|---|
| Korean (Korea) | Complete (PDF) |
This application note evaluates the ability to detect changes in ERK/CREB phosphorylation levels post treatment with Aβ using the SH-SY5Y neuroblastoma cell line.
- Publication Part Number: 5994-2527EN
- Created: 01 Feb 2021
- 2 MB
Three-dimensional (3D) in vitro cell culture platforms are motivated by the need to work with cellular models that better recapitulate the cellular microenvironment.
- Publication Part Number: 5994-2571EN
- Created: 01 Feb 2021
- 1 MB
| Korean (Korea) | Complete (PDF) |
|---|---|
| Chinese (Simplified) (China) | Complete (PDF) |
Agilent BioTek microplate readers and Take3 microvolume plate enable rapid indication of protein contamination in nucleic acid isolates using the A260/A280 ratio.
- Publication Part Number: 5994-2538EN
- Created: 01 Feb 2021
- 279 KB
This application note describes the use of the EL406 in conjunction with BioStack and LHC software to semi-automate a melamine ELISA.
- Publication Part Number: 5994-2689EN
- Created: 01 Feb 2021
- 165 KB
This application note describes the 96-well Oris cell migration assay and its use for screening test compounds that may stimulate or inhibit cell migration.
- Publication Part Number: 5994-2396EN
- Created: 01 Feb 2021
- 2 MB
This application note describes an automated microscopy and analysis protocol as an alternative method for AFB scoring.
- Publication Part Number: 5994-2546EN
- Created: 01 Feb 2021
- 462 KB
This study demonstrates the utility of a 3D spheroid cell culture model, used to elucidate the apoptotic potential of two compounds in two different cell lines.
- Publication Part Number: 5994-2591EN
- Created: 01 Feb 2021
- 471 KB
This application note demonstrates that zebrafish treated with ethanol have a dose-dependent increase in apoptotic cell death.
- Publication Part Number: 5994-2588EN
- Created: 01 Feb 2021
- 270 KB
This application note illustrates how the Cytation 3 can be used to image cells that have been cultured into 3D spheroids.
- Publication Part Number: 5994-2520EN
- Created: 01 Feb 2021
- 457 KB
This application note shows the use of a filter-based absorbance microplate reader for the enzymatic analysis of a member of the dehydrogenase family of proteins.
- Publication Part Number: 5994-2532EN
- Created: 01 Feb 2021
- 261 KB
This application note demonstrates the optimization and validation of cellular energy flux probes using a variety of Agilent BioTek readers and detection methods.
- Publication Part Number: 5994-2586EN
- Created: 01 Feb 2021
- 617 KB
This compendium describes quantitative imaging-based methods designed to provide objective evaluation of several variables that contribute to experimental results.
- Publication Part Number: 5994-3007EN
- Created: 01 Feb 2021
- 1 MB