Advanced GPC/SEC Software for OpenLab CDS
Unified GPC/SEC and HPLC Data Processing
- Advanced GPC/SEC Software for OpenLab CDS is a powerful add-on that brings polymer-specific analysis to your existing chromatography workflows. Easily installed and fully integrated, this advanced size exclusion chromatography software enables application-specific calibrations, macromolecule analysis, and advanced detection directly on data acquired with OpenLab CDS. <br><br>Compatible with both workstation and network-based setups, this advanced GPC/SEC software solution allows HPLC and GPC/SEC calculations on the same data files. It supports narrow and broad standard workflows, making it ideal for labs seeking streamlined, reliable macromolecular analysis without switching platforms.
- Integrated platform: Adds advanced GPC/SEC data analysis to OpenLab CDS
- Advanced light scattering (LS) functionality: Powerful LS workflows to perform size and aggregation analysis, and obtain absolute molecular weight insights via LS detection
- OpenLab CDS integration: Enhances your CDS platform with seamless GPC/SEC functionality
- Versatile calibration: Enables precise analysis using narrow or broad standard calibration
- Detector signal support: Processes any concentration detector signal for flexible data input
- Configurable deployment: Scales easily from standalone workstations to client-server environments
- Unified data processing: Performs both HPLC and GPC calculations on the same data file
Unified GPC/SEC and HPLC Data Processing
Bring polymer-specific analysis to your existing chromatography workflows.
Overview
Features
Configurable deployment
Unified data processing
Versatile calibration
Detector signal support
OpenLab CDS integration
Advanced light scattering (LS) functionality
How it works
GPC/SEC workflows integrated into Agilent OpenLab CDS software
Leverage the productivity, usability, and data integrity of OpenLab CDS for your gel permeation chromatography and size exclusion chromatography (GPC/SEC) analysis. The Advanced GPC/SEC software for OpenLab CDS builds on the powerful OpenLab CDS platform to enhance scalability and compliance support for GPC/SEC users.
Light scattering data analysis in OpenLab CDS
Access all GPC/SEC chromatograms, light scattering results, and compound details directly in a modern, powerful user interface from data acquired with OpenLab CDS. The Advanced GPC/SEC Software for OpenLab CDS supports both static and dynamic light scattering data analysis in combination with powerful instrument control of the 1260 Infinity II Multi-Angle Light Scattering Detector.
Advanced SEC, CDS-native
Agilent Advanced GPC/SEC Software for OpenLab CDS brings CDS native SEC light scattering to Agilent OpenLab CDS, faster onboarding, fewer errors, and lower complexity for biopharma labs.
Applications
SEC light scattering analysis of monoclonal antibodies with Advanced GPC/SEC Software for OpenLab CDS
Size exclusion chromatography, in combination with static and dynamic light scattering detection, enables accurate and reliable separation with molecular weight and size determination of monomer and aggregate protein species. The Advanced GPC/SEC Software for OpenLab CDS, in combination with the 1260 Infinity II Bio-SEC Multidetector System featuring dual-angle and DLS detection enables light scattering capabilities in a compliance-supporting CDS environment and provides a single platform for advanced SEC analysis of biopharmaceuticals.
mRNA analysis with MALS
The characterization of messenger ribonucleic acid (mRNA) with SEC-MALS enables the analysis of both biophysical and structural attributes of the molecules. Aggregation, molecular weight, and size can be determined to study the impact on these attributes from different conditions during production, formulation, and storage. The Advanced GPC/SEC Software for OpenLab CDS, in combination with the 1260 Infinity II Multi-Angle Light Scattering Detector and the 1290 Infinity III Bio LC System enables light scattering analysis in a compliance-supporting CDS environment and provides a single platform for advanced SEC analysis of biopharmaceuticals.
Documents & support
Education
Reproducible protein sizing and aggregation studies: How it works
Size exclusion chromatography delivers information on the identity, purity, and stability of biotherapeutic drugs such as monoclonal antibodies. These large biomolecules are highly sensitive to workflow stress that can induce variation in size and aggregation. Such effects can alter the biological activity and efficacy of a drug or cause undesirable side effects. The new Agilent 1260 Infinity II Bio-SEC Multidetector System enables accurate determination of molecular weight, size, and aggregate presence with highest reproducibility.
Design and characterization of bio-macromolecules
Size exclusion chromatography has long been the technique of choice for detecting and quantifying protein aggregation, polymers, and conjugates, and the technique can be expanded using advanced detectors such as light scattering and viscometry. This presentation will focus on the deployment of these techniques throughout the process and highlight complementary methodologies.
Method development and analytical services for GPC/SEC and macromolecules
Develop custom analytical and consulting services with our GPC/SEC, IPC, and 2D experts
Agilent GPC/SEC Analytical Services is a selection of contract analysis and method development services with consulting opportunities for all industries working with macromolecules and polymers. The services we provide give you the opportunity to outsource your molecular analysis, gain insights on how to advance your analysis and research, and work directly with our specialists to develop a suitable analytical method.
GPC/SEC analysis with conventional calibration
Agilent Analytical Services will perform contract analysis to determine the absolute molar masses of your macromolecular samples using conventional GPC/SEC
GPC/SEC analysis with light scattering/viscosity detection
Performing contract analysis on your macromolecular samples using GPC/SEC with light scattering and viscosity detection when no appropriate standards are available to derive molar masses.
GPC/SEC analysis according to standardized methods
GPC/SEC contract analysis of macromolecular samples following either standard methods provided by our customers, or existing national and international guidelines such as REACH. We also provide characterization data for product registration and can perform monograph testing.
Advanced macromolecular characterization
Contract analysis for the characterization of complex structured macromolecules. We perform analyses regarding molar mass, structure, branching, or chemical composition distribution on almost any macromolecule using a variety of methods. Deformulation of complex mixtures can be performed by 2D chromatography.
Method development services for macromolecules
GPC/SEC method development services, enabling you to collaborate with an Agilent specialist to create a macromolecular analysis method that is tailored to your analytical needs. Our specialists will also help you validate the method, transfer it to your laboratory, and can offer consulting on characterization of macromolecules.
Frequently asked questions
What are the key features of the Advanced GPC/SEC Software for OpenLab CDS?
Conventional as well as advanced GPC/SEC analysis can be done with the Advanced GPC/SEC software for OpenLab CDS. This software is fully integrated into OpenLab CDS. Calibrations for conventional analysis can be set and used to determine molar mass distribution and averages. The advanced license allows determination of absolute molecular weight and supports the analysis of size and aggregation via light scattering detection.
Does the GPC/SEC software for OpenLab also support advanced detection like light scattering?
Yes, light scattering signals of the Agilent 1260 Infinity II Multi-Angle Light Scattering Detector and the MDS LS can be acquired. Moreover, the light scattering signals can be analyzed to calculate absolute bulk molecular weight, Radius of gyration. With the DLS signal, Rh can be determined as well.
What is the benefit of GPC/SEC advanced detection?
Conventional GPC/SEC relies on calibration standards, which can be inaccurate for unknown or complex samples.
Advanced detection provides absolute molecular weight without calibration, improving accuracy and reliability. In addition, GPC/SEC advanced detection can provide structural information like size, conformation or branching.
What is dynamic light scattering?
Dynamic light scattering (DLS) measures the hydrodynamic size of particles in solution by analyzing fluctuations in scattered light. When coupled with size exclusion chromatography (SEC), DLS provides real-time size information of eluting species.
What is the difference between MALS and DLS?
Multi-angle light scattering (MALS) measures absolute molecular weight and radius of gyration by detecting light at multiple angles, ideal for large polymers and biopharmaceutical modalities. Dynamic light scattering (DLS) measures hydrodynamic radius based on particle diffusion and light intensity fluctuations, best for small particles and, for example, protein size determination.
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Advance your lab with technical training
Techniques of Gel Permeation and Size Exclusion Chromatography
This training course provides lectures and laboratory sessions for modern analysis of macromolecules using GPC/SEC.
Viscometry and Light Scattering Detection Methods
This course covers aspects of advanced detection methods and instruments for determination of molar masses, sizes, and structures of macromolecules
GPC/SEC Operation - 4 Day
This course covers aspects of advanced detection methods and instruments for determination of molar masses, sizes, and structures of macromolecules
Agilent GPC Operation - 1 Day
Learn the basic operation of Agilent GPC/SEC liquid chromatography and GPC/SEC software.
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