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Mitochondrial Respiration and the Seahorse XF Cell Mito Stress Test

The Gold Standard Assay for measuring mitochondrial respiration

Functional mitochondrial measurements are key to understanding cellular activation, proliferation, differentiation, and dysfunction. Researchers are using this vital information to advance their understanding of cell physiology, disease pathology, and etiology.

What parameters does the Seahorse XF Cell Mito Stress Test measure?

By directly measuring the oxygen consumption rate (OCR), the Seahorse XF Cell Mito Stress Test provides a standard and comprehensive method to assess the key parameters of mitochondrial function in both live cells and isolated mitochondria: Basal respiration, ATP-linked respiration, H+ (Proton) Leak, Maximal Respiration, Spare Respiratory Capacity, and Non-mitochondrial respiration.

How does the Seahorse XF Cell Mito Stress Test work?

The Seahorse XF Cell Mito Stress Test Kit uses modulators of cellular respiration that specifically target components of the electron transport chain (ETC) to reveal key parameters of metabolic function. The compounds, oligomycin, FCCP, and a mix of rotenone and antimycin A, are serially injected to measure ATP-linked respiration, maximal respiration, and non-mitochondrial respiration, respectively. Proton leak and spare respiratory capacity are then calculated using basal respiration and these parameters.

How can I analyze the data?

The Seahorse XF Cell Mito Stress Test Report Generator is the recommended tool to analyze data from both the Seahorse XF and XFp Cell Mito Stress Tests. This dedicated XF Report Generator automatically calculates and provides customizable summary bar graphs based on the kinetic data generated during the assay for each parameter of the XF Cell Mito Stress Test. The XF Cell Mito Stress Test Report Generator is a valuable analysis tool to view, compare, and understand the fundamental parameters of mitochondrial respiration.

How would I use it in my research?

Metabolism is inextricably linked to, and may drive, many pathologies, as well as normal cellular functions. Therefore, metabolic changes can have tremendous impact on cellular systems. The Seahorse XF Cell Mito Stress Test can help quantify multiple parameters of mitochondrial respiration and identify mitochondrial dysfunction.
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The fundamental parameters of mitochondrial function: basal respiration, ATP turnover, proton leak, maximal respiration and spare respiratory capacity.
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For Research Use Only. Not for use in diagnostic procedures.