DNF-477-0500
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Fragment Analyzer DNA/NGS Kits

Part Number:
DNF-477-0500
RUO

HS Small Fragment Kit, 500. Fragment Analyzer system kit for the quantitative and qualitative analysis of small DNA fragments and smears from 50 to 1500 bp at low concentrations. Includes the reagents necessary for running 500 samples

For Research Use Only. Not for use in diagnostic procedures.
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List Price:
$1,686.00 /1 Kit

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Specifications
Assay Sensitivity
  • High
DNA Sizing Range
  • 50-1500 bp
Fragment Sensitivity Range
  • 5–500 pg/µL
Guaranteed Shelf Life
  • Minimum 4 months
Kit Type
  • Quantitative
Quantitative Accuracy
  • ± 25 %
Quantitative Precision
  • 15 % CV
Sample Volume
  • 2 µL
Sizing Accuracy
  • ± 5 %
Sizing Reproducibility
  • 2 % CV
Sizing Resolution
  • Short Array: 50–900 bp
  • Ultrashort Array: 50–700 bp
Smear Sensitivity Range
  • 100-5000 pg/µL
Uses Per Kit
  • 500
Kit Contents
Part Number Product Description Quantity
DNF-230-0240 Small Fragment Separation Gel, 240mL 1
DNF-300-0008 BF-25 Blank Solution, 8mL 1
DNF-355-0125 5x 930 dsDNA Inlet Buffer, 125mL 1
DNF-371-0003 HS Small Fragment Diluent Marker, 2.4 mL 5
DNF-372-U100 HS Small Fragment DNA Ladder, 100uL 1
DNF-475-0050 5x Capillary Conditioning Solution, 50mL 1
DNF-497-0125 0.25x TE Rinse Buffer, 125mL 1
DNF-600-U030 Intercalating Dye, 30 µL 1
Support
User Manuals

Agilent DNF-477 HS Small Fragment Kit Quick Guide for Fragment Analyzer Systems

This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The HS Small Fragment kit is designed for sizing and quantification of dsDNA smears/fragments between 50 bp and 1,500 bp. Example applications include quality control of Next Generation Sequencing (NGS) libraries or quantitative PCR fragment analysis.

  • User Manuals
  • English
  • 04-Jul-2022
  • 227.10 Kb
  • PDF

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