GX24-2AB
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AdvanceBio Gly-X Technology

Part Number:
GX24-2AB

AdvanceBio Gly-X N-glycan prep with 2-AB Express, 24-ct. Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by on-matrix Express on-matrix labeling of released N-glycans with 2-AB dye, with no prior drydown. With deglycosylation carried out in solution, the method is simple, rapid, and suitable for automation. After a simple cleanup step, the 2-AB-labeled glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS and other methods. Includes GX24-100 Gly-X deglycosylation module, GX24-401 Gly-X 2-AB Express labeling module, GX96-402 Gly-X 2-AB Express cleanup module (96-ct).

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Specifications
Application
  • Flexible, high-throughput format: process 1 to 24 samples
Count
  • 24
Deglycosylation Efficiency
  • > 99 %
Equipment & Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)
  • DI water
  • THERM-1000, 230V)
  • Thermocycler (Corning THERM-1001, 110V
  • Vacuum manifold (Millipore MSVMHTS00-HTS)
  • Vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB Starter Kit - included free of charge with first Gly-X order, please contact us for details)
  • Vacuum pump (Millipore WP6211560, 110 V
  • WP6122050, 220V
  • Welch WOB-L Pump 2522)
Kit Configuration
  • Sample Prep Kit
Label
  • 2-AB
Precision (well-to-well, day-to-day)
  • <5% CV
Prep time (approx)
  • ~2.5 hours
Preservation of Sialylated Species
  • > 99 %
Protein Load Range
  • 1-40 µg
Recovery
  • > 80 %
Stability
  • At least 1 month at -20°C, 5 days at 4–8°C
Technique
  • LC & LC/MS
Kit Contents
Part Number Product Description Quantity
GX24-100 AdvanceBio Gly-X deglycosylation module, 24-ct 1
GX24-401 AdvanceBio Gly-X 2-AB Express labeling module, 24-ct 1
GX96-402 AdvanceBio Gly-X 2-AB Express cleanup module, 96-ct 1
Literature
Applications

Development of a Rapid 2-AB Sample Preparation Workflow for N-Glycans

The characterization of N-glycans is essential to the development of biotherapeutics.

  • Applications
  • English
  • 21-May-2020
  • 601.12 Kb
  • PDF

Preparation of Released N-Glycan Samples from Monoclonal Antibodies Using Agilent AdvanceBio Gly-X 2-AB Express for LC-Fluorescence Analysis

A method using the Agilent AdvanceBio Gly-X 2-AB Express kit and 2-AB labeling for the release of glycans with LC/FLD analysis.

  • Applications
  • English
  • 16-Sep-2019
  • 880.59 Kb
  • PDF

Streamlined Workflows for N-Glycan Analysis of Biotherapeutics with LC/FLD/MS

Streamlined workflows for N-Glycan analysis of biotherapeutics using Agilent AdvanceBio Gly-X InstantPC and 2-AB Express sample preparation with LC/FLD/MS

  • Applications
  • English
  • 21-Sep-2021
  • 1.19 Mb
  • PDF
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