GX24-401
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AdvanceBio Gly-X Technology

Part Number:
GX24-401

AdvanceBio Gly-X 2-AB Express labeling module, 24-ct (formerly ProZyme). Utilizes 2-AB (2-aminobenzamide) dye to derivatize released glycans. The proven 2-AB dye assures data continuity for ongoing projects.

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Specifications
Equipment and Reagents Provided by User
  • Two dry block heaters with 96 well PCR heat blocks. One of the heaters can be substituted with a 96-well thermocycler. Denaturation and deglycosylation can be performed using a thermocycler. A heat block is required for the labeling step.
  • Two Thermo Scientific 88871001 Compact dry block heaters with 96 well PCR heat blocks.
  • Gly-X vacuum manifold spacer and heater block lid (included in GX400 Gly-X 2-AB Express starter kit, or use GX100 spacer and alternate heater lid)
  • Vacuum manifold (Millipore MSVMHTS00)
  • Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220 V. Welch WOB-L Pump 2522)
  • DI water, ~100 µl per sample
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended), ~4 mL per sample
Stability
  • At least 1 month at -20°C, 5 days at 4–8°C
Protein Load Range
  • 1-40 µg
Label
  • 2-AB
Prep time (approx)
  • 130 min (24 samples)
Count
  • 24
Technique
  • LC & LC/MS
Kit Configuration
  • Sample Prep Module
Application
  • Reductive amination 2-AB labeling chemistry without dry down
Deglycosylation Efficiency
  • > 99 %
Recovery
  • > 80 %
Resources

GX24-401, GLY-X 2-AB EXPRESS LABELING MODULE (24-ct), 675 003, Certificate of Analysis

GX24-401, GLY-X 2-AB EXPRESS LABELING MODULE (24-ct), 675 003, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 209.51 Kb
  • PDF

GX24-401, GLY-X 2-AB EXPRESS LABELING MODULE (24-ct), 675 001, Certificate of Analysis

GX24-401, GLY-X 2-AB EXPRESS LABELING MODULE (24-ct), 675 001, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 198.00 Kb
  • PDF

GX24-401, GLY-X 2-AB EXPRESS LABELING MODULE (24-ct), 675 002, Certificate of Analysis

GX24-401, GLY-X 2-AB EXPRESS LABELING MODULE (24-ct), 675 002, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 215.12 Kb
  • PDF

GX24-401, GLY-X 2-AB EXPRESS LABELING MODULE (24-ct), 675 002, Certificate of Analysis

GX24-401, GLY-X 2-AB EXPRESS LABELING MODULE (24-ct), 675 002, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 209.66 Kb
  • PDF