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TGF-β/SMAD signaling is quantitatively evaluated in biochemical, cellular, and spheroid-based assays using a cancer model of epithelial-to-mesenchymal transition.
Rigorously validated antibody reagents from Cell Signaling Technology, combined with the multifunctional capabilities of the Agilent BioTek Cytation C10 confocal imaging reader, enable robust, quantitative analysis of TGF-β and BMP pathway...
This document is an information guide and checklist prepared for you that outlines the supplies, space, and utility requirements for the system set up in your lab.
2025 ARC Award for Studying the Connectivity Between Epigenetic Factors and Metabolism to Support Biomarker or Therapeutic Development in Neurodegenerative Diseases. The Agilent Research Catalyst (ARC) program accelerates the advancement of...
Innovative approaches to improve in vivo efficacy. LabX eBook
8850 Site Preparation
8850 Site Preparation
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Customer Responsible Site Preparations Site Preparation Checklist and Site Preparation Guide Ensure that the appropriate installation hardware has been acquired. Ensure that the location in which the GC system is being installed meets the requirements for environmental conditions. Prepare bench space for the GC system. Ensure that the bench has the size and weight capacity to accommodate the GC and associated components. Ensure that system components are oriented so that they can be connected properly. Ensure that appropriate venting is provided for the GC system. Ensure that a dedicated power circuit is available for each device in the system. Ensure that appropriate gas and reagent supplies are provided for the GC system. Ensure that appropriate gas plumbing is provided for the GC system. If the GC system being installed includes a data system, ensure that the PC meets the requirements necessary to properly support the GC system. For more information, see the site prep guide for your data system. If the GC being installed is to be connected to a site LAN, ensure that the appropriate cabling is available. Typical System Layout
CrossLab Start Up Services Agilent 6500 Series LC/QTOF and Agilent 6200 Series LC/TOF Site Preparation Video
CrossLab Start Up Services Agilent 6500 Series LC/QTOF and Agilent 6200 Series LC/TOF Site Preparation Video
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Thank you for choosing Agilent Technologies. In this video, we guide you through the CrossLab Start Up Site Preparation Checklist for the Agilent 6500 Series LC/QTOF and Agilent 6200 Series LC/TOF systems. This video serves as a comprehensive guide, outlining the necessary supplies, space, and utility requirements for an effective lab setup. Proper site preparation is crucial for ensuring that your Agilent 6500 Series LC/QTOF and/or 6200 Series LC/TOF system operates reliably for years to come. Join us to ensure a smooth installation and start realizing the full potential of your new instrument. Contact Us | Agilent Additional Resources Agilent 6200 Series LC/TOF Site Preparation Checklist Agilent 6500 Series LC/QTOF Site Preparation Checklist Chromatography & Spectroscopy Lab Supplies | Agilent LC/MS Quiet Cover, Vacuum Pump Supplies, LC/MS Supplies & Accessories | Agilent
Collection of Cell Analysis Resources
Collection of Cell Analysis Resources
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Collection of Cell Analysis Resources See also the new Cell Analysis FAQs and Troubleshooting Seahorse Overall: Cell Analysis FAQs and Troubleshooting Agilent Seahorse XF Media Selection Guide Seahorse XF Assay Learning Center Preparation of XF Assay Media Publications with Agilent Cell Analysis Data Knowing your Cells of Interest: Advice and Suggestions for a Successful XF Experience Characterizing Your Cells Using OCR Values to Determine Optimal Seeding Density Agilent Seahorse Software Specifications XFp and/or HS Mini Instrument User Guides XFp Extracellular Flux Analyzer User Guide Seahorse XF HS Mini Extracellular Flux Analyzer User Manual XFp and HS Mini Kits – User Guides Agilent Seahorse XFp Real-Time ATP Rate Assay Kit Agilent Seahorse XFp Cell Mito Stress Test Kit Agilent Seahorse XFp Glycolytic Rate Assay Kit Agilent Seahorse XFp Mito Fuel Flex Test Kit Agilent Seahorse XF Palmitate-BSA FAO Substrate Guide Agilent Seahorse XF Plasma Membrane Permeabilizer (PMP) Guide Agilent Seahorse XFp Glycolysis Stress Test Kit Agilent Seahorse XFp T Cell Metabolic Profiling Kit Site Preparation Checklist XFp Site Preparation Checklist HS Mini Site Preparation Checklist Overall User Guides XFp: Cell Characterization: The XFp Analyzer and the XF Real-Time ATP Rate Assay Seeding Adherent Cells in Agilent Seahorse XFp Cell Culture Miniplates How to Hydrate an Agilent Seahorse XFp Sensor Cartridge Washing Adherent Cells in Agilent Seahorse XFp Cell Culture Miniplates Seeding Suspension Cells in Agilent Seahorse XFp Cell Culture Miniplates Loading the Agilent Seahorse XFp Sensor Cartridge Injection Ports HS Mini: Cell Characterization: The XF HS Mini Analyzer and the XF Real-Time ATP Rate Assay Cell Characterization: The XF HS Mini Analyzer with the HS Miniplate and the XF Real-Time ATP Rate Assay Seeding Adherent Cells in Agilent Seahorse XF HS Miniplates Seeding Suspension Cells in Agilent Seahorse XF HS PDL Miniplates How to Hydrate an Agilent Seahorse XFp Sensor Cartridge Loading the Agilent Seahorse XFp Sensor Cartridge Injection Ports XF/XFe/XF Pro XFe Extracellular Flux Analyzer User Guide (Version 2.6) Agilent Seahorse Wave Pro & XF Pro Controller For use with Agilent Seahorse XF Pro Analyzers Software User Guide XFe and XF Pro Kits – User Guides Agilent Seahorse XF Real-Time ATP Rate Assay Kit Agilent Seahorse XF T Cell Metabolic Profiling Kit Seahorse XF Substrate Oxidation Stress Test Kits User Guide Agilent Seahorse XF Palmitate-BSA FAO Substrate Quickstart Guide Agilent Seahorse XF Cell Mito Stress Test Kit Agilent Seahorse XF Glycolytic Rate Assay Kit Agilent Seahorse XF Mito Fuel Flex Test Kit Agilent Seahorse XF Palmitate-BSA FAO Substrate Guide Agilent Seahorse XF Plasma Membrane Permeabilizer (PMP) Guide Agilent Seahorse XF Glycolysis Stress Test Kit XF Pro Specific Kits – User Guides ( Only for use with XF Pro) Agilent Seahorse XF Mito Tox Assay Kit User Guide Site Preparation Checklists XFe96 Site Preparation Checklist XFe24 Site Preparation Checklist XF Pro Site Preparation Checklist Normalization – User Guides Seahorse XF Imaging and Normalization System XFe24 Hoechst33342 Staining Optimization Guidelines Seahorse XF Imaging and Normalization System XFe96/XF Pro Hoechst33342 Staining Optimization Guidelines Hydration – User Guides How to Use the Agilent Seahorse XF Hydrobooster to Hydrate an Agilent Seahorse XFe96/XF Pro Sensor Cartridge How to Use the Hydro Booster for Improved Hydration and Data Quality on the Agilent Seahorse XFe 24 Analyzer Loading Injection Ports – User Guides Loading the Agilent Seahorse XFe96/XF Pro Sensor Cartridge Injection Ports Loading the Agilent Seahorse XFe24 Sensor Cartridge Injection Ports Wave Software Agilent Seahorse XFe96 and XFe24 Analyzers (For use with Wave Desktop 2.4) User Guide Wave Desktop 2.4 ReadMe Wave 2.4 Release Notes Wave 2.6 User Guide For use with Wave Desktop software and Agilent Seahorse XFe Analyzers Wave Desktop 2.6 Read Me Running Wave Desktop 2.6 on a Mac Download Hypoxia License Installer Wave Pro Software Agilent Seahorse Wave Pro & XF Pro Controller For use with Agilent Seahorse XF Pro Analyzers Agilent Seahorse XF Pro Controller ReadMe Installation Guide Agilent Seahorse XF Pro Controller Network Setup Guideline The Data Quality Analysis View in Agilent Seahorse Wave Pro and Agilent Seahorse Analytics software Automation Design Document for the Agilent Seahorse XF Pro Analyzer Articles and Whitepapers: Agilent Seahorse Analytics Security Version 2.0.1 Improving Quantification of Cellular Glycolytic Rate Using Agilent Seahorse XF Technology Quantifying Cellular ATP Production Rate Using Agilent Seahorse XF Technology Technical Overviews 3T3-L1 Pre-Adipocyte Differentiation Assessing Mitochondrial Respiratory Complexes Using Cells Permeabilized with XF Plasma Membrane Permeabilizer (PMP) Automation Design Document for the Agilent Seahorse XF Pro Analyzer Conducting an XFe Assay in an Hypoxia Chamber at ≥3% O² * Data Quality Management using Brightfield Images with the Seahorse XF Imaging and Normalization System Evaluation of Edge Effect & Evaporation from Agilent Seahorse XF Pro M Culture Plates Explore Metabolism Where You Couldn’t Before with Seahorse XF HS Mini Analyzer Hoechst Staining Optimization Technical Guidelines - XFe24 Analyzer Hoechst Staining Optimization Technical Guidelines - XFe96 Analyzer Normalization Technical Guidelines Immobilization of Non-Adherent Cells with Cell-Tak for Assay on the Agilent Seahorse XFe/XF96 or XFp Analyzer Application Notes Immobilization of Non-Adherent Cells with Cell-Tak for Assay on the Agilent Seahorse XFe/XF24 Analyzer Islet Assay Using the Agilent Seahorse XF24 Islet Capture Microplate Methods and strategies for normalizing XF metabolic data to cellular parameters Modifying Assay Guides for use with Agilent Seahorse XFe24 and XF24 Analyzers Screening for Mitochondrial Toxicity - A Protocol for the isolation of mitochondria and measurement Assessing Mitochondrial Respiratory Complexes Using Cells Permeabilized with XF Plasma Membrane Permeabilizer (PMP) 3T3-L1 Pre-Adipocyte Differentiation Using the XF Plasma Membrane Permeabilizer (PMP) to Measure Substrate Oxidation Using PMP to Measure Mitochondrial ETC Complex Activity in Limited Biomaterials Validation of a Six-hour Seahorse XF assay on the Agilent Seahorse XF Pro analyzer Posters A Novel Solution to Assess T Cell Metabolic Phenotype for the Optimization of Antitumor T cell Therapy Products Assessment of drug-induced liver mitochondrial dysfunction in primary human hepatocytes using XF Mito Tox Assay Bioenergetic profiling of cancer cell lines: quantifying the impact of glycolysis on cell proliferation. Changes In Metabolic Phenotype And Cellular ATP Production During CD4+ T Cell Activation Characterization of fuel dependencies in multidrug resistant breast cancer cells A Novel Solution to Better Understand the Link Between T Cell Metabolism and Function Quality Assessment of Metabolic Fitness during T Cell and NK Cell Expansion Determining Mechanisms of Mitochondrial Toxicity using Agilent Seahorse XF Technology Determining the Metabolic Consequences of Pharmacological Modulation of T Cell Activation Metabolic discovery platform:“Seahorse-Bravo” Screening and validation of mitochondrial inhibitors and uncouplers using a novel toxicity index derived from mitochondrial oxygen consumption rates Cell-based screening of drug-induced metabolic dysfunctionHigh-throughput in vitro ischemia-reperfusion model with real-time monitoring of cellular oxygenation and reactive oxygen species generation High-throughput in vitro ischemia-reperfusion model with real-time monitoring of cellular oxygenation and reactive oxygen species generation Multiplexed Screening of Drug-Induced Mitochondrial Dysfunction and Cytotoxicity Simple High-Throughput Workflows for Assessing Drug-Induced Mitochondrial Dysfunction Using Oxygen Consumption Measurements Application Notes Application Notes (overall link) A Customized XF Workflow for Detection and Characterization of Mitochondrial Toxicity Assessing functional metabolism using cells sourced from blood Assessing Microbial Proliferation and Antibiotic Resistance using the MitoXpress Xtra Oxygen Consumption Assay Assessing mitochondrial dysfunction in primary cardiomyocytes Assessing the Mitochondrial Toxicity of Environmental Pollutants Using the Agilent Seahorse XF Extracellular Flux Analyzer Assessing NK Cell Bioenergetic Metabolism Using Extracellular Flux Analysis Assessing T Cell Bioenergetic Poise and Spare Respiratory Capacity Using Extracellular Flux Analysis Assessing the impact of autophagy on cellular metabolism Assessing therapies for traumatic brain or spinal cord injuries Bioenergetic analysis of primary neuronal deficiencies Bioenergetic analysis of suspension cells: hematopoietic stem cells and lymphocytes Cancer Cells Evoke Flexibility By Maintaining a Bioenergetic Balance Between Glycolysis and Mitochondrial Respiration Linked to Metastatic Potential Cell-Based Screening for Drug‑Induced Mitochondrial Toxicity Characterization of Glycolysis with a Panel of Common Cellular Models Using Agilent Seahorse XF Technology Defining metabolic phenotypes in cancer cells Determining Metabolic Switch in Induced Pluripotent Stem Cells (iPSCs) Direct Measurements of Cellular Metabolism for Identification of Mitochondrial Drug Targets Evaluating changes in cell metabolism in neuroimmune and neuropsychiatric disorders Functional validation of Genomic and Proteomic data Gaining Insights into Disease Biology for Target Identification and Validation using Seahorse XF Technology Identifying Metabolic Switches Using an Agilent Seahorse XFe Analyzer in Hypoxia Maximizing Efficiency and Performance of Seahorse XF Assays with the Bravo Automated Liquid Handling Platform Measurement of Mitochondrial Toxicity Using Primary Hepatocytes Measuring bioenergetic profiles of human adipocytes Measuring functional metabolism of brite adipocytes Measuring glycolysis and oxidative metabolism in cancer cells Measuring glycolytic function in cells Measure Mitochondrial Respiration in Skeletal Muscle Fibers with a Seahorse Analyzer Measuring the metabolic switch in cancer cells Metabolic Pathway Determination Using the Seahorse XF24 Analyzer Normalization of Agilent Seahorse XF Data by In-situ Cell Counting Using a BioTek Cytation 5 Quantitative Analysis of Neutrophil Activation Using Agilent Seahorse XF Technology Rapid Bioenergetic Functional Screening of Anticancer Drug Candidates Real Time Discrimination of Inflammatory Macrophage Activation Using Agilent Seahorse XF Technology Revealing Cellular Metabolic Phenotype and Function Using Agilent XF Substrate Oxidation Stress Tests Using the Agilent NovoCyte Flow Cytometer for Immune Suspension Cell Normalization in Agilent Seahorse XF Assays XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System Quick Reference Guides Quick Reference Guide: Calculating Proton Efflux Rate (PER) Data Agilent Seahorse XF Buffer Factor Protocol Quick Reference Guide Informative and Instructional Videos Cell Analysis Video Library (Overall) Analyze Agilent Seahorse XF Real-Time ATP rate assay data using Agilent Seahorse Analytics How to accelerate your analysis workflow with Agilent Seahorse Analytics How to transform data into insight with Agilent Seahorse Analytics How to keep your data organized and collaborate more efficiently with Agilent Seahorse Analytics Seahorse XF Mito Tox Assay with Seahorse Analytics Analyze Agilent Seahorse XF Hu T Cell Activation Assay data using Agilent Seahorse Analytics software Analyze Agilent Seahorse XF Substrate Oxidation Stress Test data using Agilent Seahorse Analytics Bravo Automation-XF Assay Demonstration Bravo Automation-XF Assay Streamline Cell Analysis Normalization System Overview Cell Energy Pathways How to analyze XF neutrophil activation result data How to accelerate your analysis workflow with Agilent Seahorse Analytics How to keep your data organized and collaborate more efficiently with Agilent Seahorse Analytics How to transform data into insight with Agilent Seahorse Analytics Measuring Cell Metabolism in Transient Microchamber Seahorse XF Mito Tox Assay with Seahorse Analytics Seahorse Analyzer Multi Port Drug Injection Seeding Cell Culture Microplates XF Real-Time ATP Rate Assay Webinars Webinars (overall link) Report Generators – Single and Multi-file (if available) Seahorse XF Cell Mito Stress Test Report Generators Seahorse XF Glycolytic Rate Assay Report Generators Seahorse XF Mito Fuel Flex Test Report Generator Seahorse XF Real-Time ATP Rate Assay Report Generators Soluble Sensors Overall: Plate Reader Compatibility MitoXpress Xtra Oxygen Consumption Assay Plate Reader compatibility chart Ph-Xtra Glycolysis Assay Plate Reader compatibility chart MitoXpress Intra Plate Reader Compatibility Guide Instrument Setup Guides – MitoXpress Xtra Oxygen Consumption Assay Setup Guides MitoXpress Xtra Oxygen Consumption Assay Instrument setup guide for Perkin Elmer plate readers MitoXpress Xtra Oxygen Consumption Assay Instrument setup guide for Tecan plate readers MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for BMG Plate Readers MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for Molecular Devices Plate Readers MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for BioTek Plate Readers Instrument Setup Guides – pH-Xtra Glycolysis Assay Setup Guides pH-Xtra Glycolysis Assay Instrument setup guide for Perkin Elmer plate readers pH-Xtra Glycolysis Assay Instrument setup guide for Tecan plate readers pH-Xtra Glycolysis Assay Instrument Set Up Guide for BioTek Plate Readers pH-Xtra Glycolysis Assay Instrument Set Up Guide for Molecular Devices Plate Readers pH-Xtra Glycolysis Assay Instrument Set Up Guide for BMG Plate Readers User Manuals and Technical Overviews Agilent pH-Xtra Glycolysis Assay Agilent MitoXpress Xtra Oxygen Consumption Assay (HS Method) Agilent MitoXpress Intra Intracellular Oxygen Assay Agilent Mito-rOCR Assay Kit User Guide Mito-rOCR Gen5 and Demo Files Improved Assay Performance and Workflow with Agilent Mito-rOCR Compared to Oil-Based OCR Assays Application Notes and Posters Assessing the Impact of Drug Treatment on Cardiomyocyte Function Assessing Microbial Proliferation and Antibiotic Resistance using the MitoXpress Xtra Oxygen Consumption Assay Metabolic Profiling of Cells in 3D Cultures using MitoXpress Xtra and pH-Xtra Assays Screening for Drug-Induced Mitochondrial Dysfunction Using MitoXpress Xtra on Isolated Mitochondria Multiplexed Assessment of Mitochondrial Function Combining the Agilent Mito-rOCR Assay with Live-Cell Imaging Multiplexed assessment of mitochondrial function with real-time oxygen consumption measurement and live cell image analysis How-To Videos How Mito-rOCR works Agilent Mito-rOCR Data Analysis Agilent Mito-rOCR Product Innovations Software Data Visualization Tool Agilent MitoXpress & pH-Xtra Data Visualization Tool User Manual Have a question?
Our Favorite Resources to Get Started with Your New GC or GC/MS System
Our Favorite Resources to Get Started with Your New GC or GC/MS System
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A compilation of our favorite resources to help you get started with your new GC or GC/MS System: Free Agilent Education courses are available to guide you through site preparation of GC systems including systems with a Mass Spectrometer, Headspace Sampler, and 7693 ALS. The course goes into detail about space, weight, environmental conditions, ventilation, exhaust, power, gas, and more: For 8890 GCs: GC-8890-1201e - Getting Prepared for Your New GC System For Intuvo 9000 GCs: GC-9000-1201e - Preparing for Your New Agilent Intuvo 9000 GC A free Gas Management course is also available: GC-0GEN-1001e - Gas Management Best Practices Recommended Learning Paths for GC and GC/MS Systems are available: Learning Pathways The Agilent 5973, 5975, and 5977 Series GC/MSD Supplies quick reference guide – consumables you should know about for the MSD: Agilent 5973, 5975, and 5977 Series GC/MSD Supplies Site Preparation Guides or Checklists 7890 Series GC Site Preparation Checklist 8860 GC Site Preparation Guide 8890 GC Site Preparation Guide Intuvo 9000 GC, GC/MS, and ALS Site Preparation Guide 5977B Series MSD Site Preparation Checklist 7000 and 7010 Series System Site Preparation Checklist More Resources Your GC Productivity Roadmap Essential Chromatography and Spectroscopy Catalog Series
Drug-induced cardiac toxicities remain a leading cause of drug attrition and FDA black box warnings, highlighting the need for predictive assessment strategies.
The water immersion capability of the Cytation C10 confocal imaging reader enables gentler imaging by minimizing light exposure, reduces Z-distortion of 3D biological samples, and is conducive to high-throughput applications.
This application brief shows how the Agilent BioTek Cytation C10 confocal imaging reader improves the resolution of mouse retinal vasculature, compared to widefield microscopy.
This application brief demonstrates the use of the Auto ROI feature of Gen5 to identify mitotic cells for further characterization using confocal microscopy.
Efficiently screen for inhibitors of MT1-MMP activity and 3D glioma tumoroid invasion using a fluorescent substrate-based MMP activity assay and hit pick reading/imaging procedure.
In this application note, the Agilent BioTek Cytation 5 cell imaging multimode reader was used for quantitative assessment of antioxidant activities in living cells.
In this application note, chloroquine was used to demonstrate that LUCS assay can be implemented in a “plug-and-play” mode using the Agilent BioTek Cytation 5.
G5578GA, G5579GA Bravo BenchCel DB Workstation Site Preparation Checklist
This document is an information guide and checklist prepared for you that outlines the supplies, space, and utility requirements for the system set up in your lab.
A streamlined neurotoxicity solution that combines single-step, live-cell staining with an automated, high-throughput imaging and analysis platform.
Automated viral infectivity application leveraging Agilent BioTek Cytation automated live-cell imaging and the AVIA AI-based analysis platform from ViQi, Inc.
Introducing an automated neurite analysis module for Agilent BioTek imaging systems, with iPSC-derived and primary neuron culture model evaluations.
Several colorimetric and fluorescent assays are described incorporating automated image acquisition and analyses using Agilent’s high-content imaging instruments.
A technical overview of the Agilent automation solutions supporting the most recent Cell Painting assay protocol from cell seeding to imaging.
In this webinar, we describe high-throughput methods to investigate TGF-β-induced EMT in biochemical, 2D cellular and 3D spheroid adenocarcinoma models...