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BioTek Cytation C10 Confocal Imaging Reader

Imaging reader combines automated confocal and widefield microscopy with multimode microplate reading

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The Cytation C10 confocal imaging reader combines automated confocal and widefield microscopy with conventional multimode microplate reading in a unique, proprietary design. The automated water-immersion objectives capture more light, driving lower exposure times and reducing phototoxic impacts on live cells. The spinning-disk confocal module and accompanying disk pinhole options allow a researcher to look deeper into thick sample biology with improved clarity and detail. High-quality components used in the system, including a Hamamatsu scientific CMOS (sCMOS) camera, Olympus objectives and laser-based illumination enable excellent image quality.|Cytation C10 also includes widefield fluorescence, brightfield, and phase contrast optics. Onboard environmental controls enable live cell assays. Multiplate live cell assays are accomplished with Cytation C10 integrated with the Agilent BioTek BioSpa 8 automated incubator. Cytation C10 brings high-performance, affordable confocal to every laboratory.

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MassHunter Retention Index LRI MassHunter Retention Index LRI

Hello, For my analysis, i want to use retention index confirmation with NIST MS confirmation. To do that, i injected a alkanes mix C9,C10,C12 C13 C16 C17 C18 C19. I made a batch from this injection and used NIST database to create my compound table with RI from NIST. Normally each alkane should have RI like RI = 100 Nb C From this batch, I used RT calibration and generated my RT calibration file: Nonane 111-84-2 916 12.2312 Decane 124-18-5 1015 15.3985 Dodecane 112-40-3 1214 19.2719833 Tridecane 629-50-5 1313 20.7816333 Hexadecane 544-76-3 1612 24.6508833 Heptadecane 629-78-7 1711 25.7968667 Octadecane 593-45-3 1810 26.8878667 Nonadecane 629-92-5 1910 27.91545 So I don't understand why RI from calibration file are different from theorical RI example Decane theorical 10*100 vs cal value 916 Someone can explain this difference?

I need a method for Free fatty acid analysis of GHEE on GC-FID, thanks in advance I need a method for Free fatty acid analysis of GHEE on GC-FID, thanks in advance

community.agilent.com/.../GHEE-SAMPLE-_5F00_-442306.pdf

n-Alkane standard run on GC/FID n-Alkane standard run on GC/FID

If I run a standard of n-alkanes ranging from C10-C40 on GC/FID, with each component being the same concentration, should each peak have the same area?

Setting up ASTM D5769 in MSD Chemstation Setting up ASTM D5769 in MSD Chemstation

But I have a slightly high bias on gasoline samples. I think my uncalibrated C10 benzenes may be too high and I am wondering if I have that set up properly. Does anyone know how to correctly set up the summing? I am worried that it may be including C10 benzenes that I am already separately calibrated for.

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