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The Agilent BioTek Cytation 9 cell imaging reader combines multimode microplate reading with widefield inverted and upright microscopy in one platform. This evolution of the Cytation family offers faster throughput and expanded imaging capabilities...
Fully automated live‑cell imaging with Agilent BioTek Cytation 9 and BioSpa 8 enables high‑throughput, time‑resolved analysis of cell proliferation, apoptosis, and irreversible cell death.
- Publication Part Number: 5994-9060EN
- Created: 18 Mar 2026
- 7 MB
This application note describes an automated, high-throughput approach to assay collecting and single-cell migration using quantitative imaging.
- Publication Part Number: 5994-9152EN
- Created: 29 Apr 2026
- 3 MB
This document highlights the technical details and configurations of the Agilent BioTek Cytation 9 cell imaging multimode reader.
- Publication Part Number: 5994-9000EN
- Created: 13 Mar 2026
- 135 KB
Collection of Cell Analysis Resources
Collection of Cell Analysis Resources
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Changes In Metabolic Phenotype And Cellular ATP Production During CD4+ T Cell Activation Characterization of fuel dependencies in multidrug resistant breast cancer cells A Novel Solution to Better Understand the Link Between T Cell Metabolism and Function Quality Assessment of Metabolic Fitness during T Cell and NK Cell Expansion Determining Mechanisms of Mitochondrial Toxicity using Agilent Seahorse XF Technology Determining the Metabolic Consequences of Pharmacological Modulation of T Cell Activation Metabolic discovery platform:“Seahorse-Bravo” Screening and validation of mitochondrial inhibitors and uncouplers using a novel toxicity index derived from mitochondrial oxygen consumption rates Cell-based screening of drug-induced metabolic dysfunctionHigh-throughput in vitro ischemia-reperfusion model with real-time monitoring of cellular oxygenation and reactive oxygen species generation High-throughput in vitro ischemia-reperfusion model with real-time monitoring of cellular oxygenation and reactive oxygen species generation Multiplexed Screening of Drug-Induced Mitochondrial Dysfunction and Cytotoxicity Simple High-Throughput Workflows for Assessing Drug-Induced Mitochondrial Dysfunction Using Oxygen Consumption Measurements Application Notes Application Notes (overall link) A Customized XF Workflow for Detection and Characterization of Mitochondrial Toxicity Assessing functional metabolism using cells sourced from blood Assessing Microbial Proliferation and Antibiotic Resistance using the MitoXpress Xtra Oxygen Consumption Assay Assessing mitochondrial dysfunction in primary cardiomyocytes Assessing the Mitochondrial Toxicity of Environmental Pollutants Using the Agilent Seahorse XF Extracellular Flux Analyzer Assessing NK Cell Bioenergetic Metabolism Using Extracellular Flux Analysis Assessing T Cell Bioenergetic Poise and Spare Respiratory Capacity Using Extracellular Flux Analysis Assessing the impact of autophagy on cellular metabolism Assessing therapies for traumatic brain or spinal cord injuries Bioenergetic analysis of primary neuronal deficiencies Bioenergetic analysis of suspension cells: hematopoietic stem cells and lymphocytes Cancer Cells Evoke Flexibility By Maintaining a Bioenergetic Balance Between Glycolysis and Mitochondrial Respiration Linked to Metastatic Potential Cell-Based Screening for Drug‑Induced Mitochondrial Toxicity Characterization of Glycolysis with a Panel of Common Cellular Models Using Agilent Seahorse XF Technology Defining metabolic phenotypes in cancer cells Determining Metabolic Switch in Induced Pluripotent Stem Cells (iPSCs) Direct Measurements of Cellular Metabolism for Identification of Mitochondrial Drug Targets Evaluating changes in cell metabolism in neuroimmune and neuropsychiatric disorders Functional validation of Genomic and Proteomic data Gaining Insights into Disease Biology for Target Identification and Validation using Seahorse XF Technology Identifying Metabolic Switches Using an Agilent Seahorse XFe Analyzer in Hypoxia Maximizing Efficiency and Performance of Seahorse XF Assays with the Bravo Automated Liquid Handling Platform Measurement of Mitochondrial Toxicity Using Primary Hepatocytes Measuring bioenergetic profiles of human adipocytes Measuring functional metabolism of brite adipocytes Measuring glycolysis and oxidative metabolism in cancer cells Measuring glycolytic function in cells Measure Mitochondrial Respiration in Skeletal Muscle Fibers with a Seahorse Analyzer Measuring the metabolic switch in cancer cells Metabolic Pathway Determination Using the Seahorse XF24 Analyzer Normalization of Agilent Seahorse XF Data by In-situ Cell Counting Using a BioTek Cytation 5 Quantitative Analysis of Neutrophil Activation Using Agilent Seahorse XF Technology Rapid Bioenergetic Functional Screening of Anticancer Drug Candidates Real Time Discrimination of Inflammatory Macrophage Activation Using Agilent Seahorse XF Technology Revealing Cellular Metabolic Phenotype and Function Using Agilent XF Substrate Oxidation Stress Tests Using the Agilent NovoCyte Flow Cytometer for Immune Suspension Cell Normalization in Agilent Seahorse XF Assays XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System Quick Reference Guides Quick Reference Guide: Calculating Proton Efflux Rate (PER) Data Agilent Seahorse XF Buffer Factor Protocol Quick Reference Guide Informative and Instructional Videos Cell Analysis Video Library (Overall) Analyze Agilent Seahorse XF Real-Time ATP rate assay data using Agilent Seahorse Analytics How to accelerate your analysis workflow with Agilent Seahorse Analytics How to transform data into insight with Agilent Seahorse Analytics How to keep your data organized and collaborate more efficiently with Agilent Seahorse Analytics Seahorse XF Mito Tox Assay with Seahorse Analytics Analyze Agilent Seahorse XF Hu T Cell Activation Assay data using Agilent Seahorse Analytics software Analyze Agilent Seahorse XF Substrate Oxidation Stress Test data using Agilent Seahorse Analytics Bravo Automation-XF Assay Demonstration Bravo Automation-XF Assay Streamline Cell Analysis Normalization System Overview Cell Energy Pathways How to analyze XF neutrophil activation result data How to accelerate your analysis workflow with Agilent Seahorse Analytics How to keep your data organized and collaborate more efficiently with Agilent Seahorse Analytics How to transform data into insight with Agilent Seahorse Analytics Measuring Cell Metabolism in Transient Microchamber Seahorse XF Mito Tox Assay with Seahorse Analytics Seahorse Analyzer Multi Port Drug Injection Seeding Cell Culture Microplates XF Real-Time ATP Rate Assay Webinars Webinars (overall link) Report Generators – Single and Multi-file (if available) Seahorse XF Cell Mito Stress Test Report Generators Seahorse XF Glycolytic Rate Assay Report Generators Seahorse XF Mito Fuel Flex Test Report Generator Seahorse XF Real-Time ATP Rate Assay Report Generators Soluble Sensors Overall: Plate Reader Compatibility MitoXpress Xtra Oxygen Consumption Assay Plate Reader compatibility chart Ph-Xtra Glycolysis Assay Plate Reader compatibility chart MitoXpress Intra Plate Reader Compatibility Guide Instrument Setup Guides – MitoXpress Xtra Oxygen Consumption Assay Setup Guides MitoXpress Xtra Oxygen Consumption Assay Instrument setup guide for Perkin Elmer plate readers MitoXpress Xtra Oxygen Consumption Assay Instrument setup guide for Tecan plate readers MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for BMG Plate Readers MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for Molecular Devices Plate Readers MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for BioTek Plate Readers Instrument Setup Guides – pH-Xtra Glycolysis Assay Setup Guides pH-Xtra Glycolysis Assay Instrument setup guide for Perkin Elmer plate readers pH-Xtra Glycolysis Assay Instrument setup guide for Tecan plate readers pH-Xtra Glycolysis Assay Instrument Set Up Guide for BioTek Plate Readers pH-Xtra Glycolysis Assay Instrument Set Up Guide for Molecular Devices Plate Readers pH-Xtra Glycolysis Assay Instrument Set Up Guide for BMG Plate Readers User Manuals and Technical Overviews Agilent pH-Xtra Glycolysis Assay Agilent MitoXpress Xtra Oxygen Consumption Assay (HS Method) Agilent MitoXpress Intra Intracellular Oxygen Assay Agilent Mito-rOCR Assay Kit User Guide Mito-rOCR Gen5 and Demo Files Improved Assay Performance and Workflow with Agilent Mito-rOCR Compared to Oil-Based OCR Assays Application Notes and Posters Assessing the Impact of Drug Treatment on Cardiomyocyte Function Assessing Microbial Proliferation and Antibiotic Resistance using the MitoXpress Xtra Oxygen Consumption Assay Metabolic Profiling of Cells in 3D Cultures using MitoXpress Xtra and pH-Xtra Assays Screening for Drug-Induced Mitochondrial Dysfunction Using MitoXpress Xtra on Isolated Mitochondria Multiplexed Assessment of Mitochondrial Function Combining the Agilent Mito-rOCR Assay with Live-Cell Imaging Multiplexed assessment of mitochondrial function with real-time oxygen consumption measurement and live cell image analysis How-To Videos How Mito-rOCR works Agilent Mito-rOCR Data Analysis Agilent Mito-rOCR Product Innovations Software Data Visualization Tool Agilent MitoXpress & pH-Xtra Data Visualization Tool User Manual Have a question? Ask the Cell Analysis community
Webinar Digest Update - 9/11/2024
Webinar Digest Update - 9/11/2024
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The Digest of upcoming webinars has been updated as of September 11. Notable additions include: Atomic (1) Cell Analysis (1) GC & GCMS (1) Check out the Pathology A La Carte Webinar Series! Click on the Featured Webinar for more information, or register here for the upcoming September 24 webinar: How Can Scalable, Open Digital Pathology Solutions Advance Laboratory Efficiency and Diagnostic Quality in Pathology Practice? Analysis of Major and Trace Constituents in Li-ion Battery Electrolyte Solutions using ICP-OES on September 17 Comprehensive Quality Control Testing of Biofuels to Ensure a Greener, more Sustainable Future on September 17 Metabolic Insights into Tumor and Tissue Development: Seahorse Analysis and Organoid Imaging on September 24 4th Annual Alcoholic Beverages Virtual Symposium: Spirits on September 17 Dissolution Method Development Guidance Using QbD on November 21 Tips, Tricks, and Troubleshooting for Successful Headspace Analysis in GC on September 17 HPLC-Beginner Webinar Series - #2 Stationary Phases in HPLC - Part I on September 17 UV-Vis Spectroscopy: Export Your Measurement Out of the Instrument on September 12 Back to the Lab with Your Fragment Analyzer and Femto Pulse on September 26 How Can Scalable, Open Digital Pathology Solutions Advance Laboratory Efficiency and Diagnostic Quality in Pathology Practice? on September 24 Seminar 1 – OpenLab CDS - The Control Panel and Data Acquisition on October 8 Seminar 2 – OpenLab CDS – Data Analysis on October 15 Seminar 3 – OpenLab CDS – Custom Calculator and Reporting on October 22 Seminar 4 - OpenLab CDS for MS Data on October 29 Agilent and Scitara: Enabling Customers To Get The Most Value from Lab Data on September 18 Check out the August GC Live Event On Demand: End Consumables Counting Chaos: Track and Trend for Timely GC Maintenance and learn how intelligent Agilent gas chromatography systems take Early Maintenance Feedback (EMF) to a new level. See the complete list here: Webinar Notifications A listing of past recorded webinars is available here: Agilent Recorded Webinars Thank you.
Webinar Digest Update - 4/27/2026
Webinar Digest Update - 4/27/2026
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Check out: Improve your ICP-MS productivity and workflow with the ADS2/AVS on April 30 Impurity Characterization of GLP-1 Receptor Agonists via RPLC and HILIC on May 7 Agilent BioTek Cytation 9 Technology: Orchestrating Orthogonal Cell Analysis at Scale on April 29 Heavy Metals & Speciation Edition on May 21 Green Solutions on the Agilent Micro GC on May 7 Miniaturized for Scalable NGS Genotyping: The CannSelect Platform as a Model for Cost Efficient, High Throughput Targeted Sequencing. on April 28 Navigating PFAS Complexity: Addressing the Unique Chromatographic Challenges of Ultra‑Short‑Chain PFAS on May 27 FTIR in Action: From Spectroscopy Fundamentals to Oil and Grease Analysis in Water on April 28 Future-Ready Pharma Summit on May 12 Seminar 4 - OpenLab CDS for MS Data on April 28 See the complete list here: Webinar Notifications A listing of past recorded webinars is available here: Agilent Recorded Webinars Thank you.
Webinar Digest Update - 4/3/2026
Webinar Digest Update - 4/3/2026
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Check out: Accelerating GLP-1 Analog Impurity Profiling with the Agilent Pro IQ Plus Followed by Detailed Sequence Analysis using Electron Capture Dissociation on April 16 Agilent BioTek Cytation 9 Technology: Orchestrating Orthogonal Cell Analysis at Scale on April 29 Pesticide Residues & Fumigants Edition on April 23 Column Confidence: Making the Right Choice for Your GC Needs on April 16 Quality Time - Right Tools, Real Results on April 28 Getting the Most from Your Agilent LCMS System on April 21 Seminar 1 - OpenLab CDS - The Control Panel and Data Acquisition on April 7 Seminar 2 – OpenLab CDS – Data Analysis on April 14 Seminar 3 – OpenLab CDS – Custom Calculator and Reporting on April 21 Seminar 4 - OpenLab CDS for MS Data on April 28 See the complete list here: Webinar Notifications A listing of past recorded webinars is available here: Agilent Recorded Webinars Thank you.
Discover the Agilent BioTek Cytation 9, merging multimode microplate reading with widefield inverted and upright microscopy for faster, data-driven insights.
- Playing Time: 00:04:11
Cytation multimode reader bridges the gap between high-throughput screening and deep mechanistic insight by uniting powerful imaging and quantitative data.
- Playing Time: 00:04:52
Commonly asked questions and answers about Agilent BioTek cell imaging multimode readers, automated microscopes and software for imaging & microscopy.
Commonly asked questions and answers about Agilent BioTek microplate readers, including hybrid multimode readers, multimode readers, and absorbance readers.
Agilent BioTek automated cell imagers and microscopes bring your science to life, capturing spectacular images, z-stacks, montages and time lapse sequence with ease.
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Cytation Hybrid cell imaging multimode readers with Hybrid Technology for sensitivity and flexibility
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A core component for performing reproducible and sensitive metabolic measurements
View informative Agilent Cell Analysis Technology webinars on a variety of research subjects.
Fixed samples for fluorescence microscopy applications can take many shapes and forms, including cultured cells in microplates, multicellular spheroids, tissue on slides, and whole organisms.
A comprehensive understanding of the physiological state or health of your cells is vitally important for achieving reliable experimental outcomes, as results are highly dependent on the initial condition of the cells.
Review past issues of TekTalk, Agilent‘s complimentary cell analysis life science instrumentation e-newsletter. If you are not currently subscribed, you may sign up now to begin receiving issues as they are released. Our current distribution is 6...
Review past issues of TekTalk, Agilent‘s complimentary cell analysis life science instrumentation e-newsletter. If you are not currently subscribed, you may sign up now to begin receiving issues as they are released. Our current distribution is 6...
This application note demonstrates an automated γH2AX assay workflow using a novel cell imaging multimode reader with advanced data analysis capabilities.
- Publication Part Number: 5994-2594EN
- Created: 27 Jan 2023
- 2 MB
This application note presents a method to capture and project multiple single z-plane images using digital widefield microscopy.
- Publication Part Number: 5994-3349EN
- Created: 09 Aug 2022
- 2 MB