Reliable, Robust Solutions to Advance Your Analysis of Oligonucleotides

Due to their potential to treat a wide range of diseases, oligonucleotides have emerged as an area of particular interest.

Like all therapeutics, synthetic oligonucleotide therapeutics, such as antisense oligonucleotides (ASOs) and small interfering RNAs (siRNAs), require thorough analysis to establish intended purity and quality.

Vetter Pharma, a contract development and manufacturing organization (CDMO) headquartered in Ravensburg, Germany, supports the production processes of their biotechnology and pharmaceutical customers through analytical services such as quality control (QC) testing for incoming goods, in-process and filled drug products, stability studies, and developmental analytics.

“One of our projects aims to optimize the analytics of oligonucleotide-based pharmaceuticals,” said Dr. Melanie Zerulla-Wernitz, head of the Analytical Science Laboratory at Vetter.

Vetter applies UV-Vis technology with next-generation sample temperature control using an Agilent Cary 3500 spectrophotometer for their oligonucleotide identification tests in accordance with GMP regulations. “There is a trend towards using melting temperature (T_m) in combination with intact mass measurement, combining sequence and mass to achieve unequivocal identification,” explains Dr. Zerulla-Wernitz. “For this approach, the Cary 3500 UV-Vis provides state-of-the-art technology. While there are other possibilities, we are convinced that a combination of measuring T_m and mass will be the standard analytical strategy for QC ID testing in the future,” she remarked.

In other projects, Vetter also utilizes additional Agilent solutions such as Agilent HPLC systems as well as the Agilent 7800 ICP-MS. Dr. Zerulla-Wernitz commented, “We have had a positive experience working with Agilent over the long term. Agilent has been a reliable and professional partner for service and questions; even with complicated issues, Agilent technicians were available within a short time and provided comprehensive support.”

For broader oligonucleotide analysis, Agilent supports its customers with complete workflows featuring instruments, software, columns, and consumables. In addition, we can assist you with GMP manufacturing of late-stage and commercial quantity oligonucleotides.