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Advance BioHPLC Performance

Advance your confidence for biotherapeutics

Agilent AdvanceBio columns are designed to enhance the accuracy and speed of your biomolecule characterization, including monoclonal antibodies (mAbs), other proteins, peptides and synthetic oligonucleotides.

Featured Product: NEW Agilent AdvanceBio Desalting-RP cartridges

For Research Use Only. Not for use in diagnostic procedures.

End uncertainty

Eliminate slow separations, poor resolution, and other mAb characterization roadblocks with the new Agilent AdvanceBio SEC columns

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Resolve mAbs faster and better

Agilent AdvanceBio RP-mAb columns provide sharper, narrower peaks for confident separations of monoclonal antibodies

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Advance your glycan mapping

Achieve reproducible, high-resolution identification of 2-AB labeled N-linked glycans with the AdvanceBio Glycan Mapping columns, sample preparation and standards

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New AdvanceBio Columns Improve Laboratory Workflows

Biotech Embroiders Intricate Glycopatterns

New eSeminars

Topic: Versatile PLRP-S Columns for a reversed phase solution for the characterization of monoclonal antibodies

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Topic: LabRoots webinar: Two Dimensional Liquid Chromatography Applied to the Characterization of Monoclonal Antibodies and Antibody-Drug Conjugates.
Presenter: Koen Sandra, Ph.D., R&D Director Life Sciences, Research Institute for Chromatography (RIC)
Time: Tuesday, October 11, 2016—11:00 am ET

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Topic: Development of analysis methods for monoclonal antibodies using innovative superficially porous particle biocolumns
Time: Thursday, November 17, 2016—11:00 am ET

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On-demand seminars

Agilent Biopharma Solutions

Moving the field of Biopharma forward

Protein Identification, Impurity Profiling, and Post Translational Modifications

Reversed-phase columns to boost your accuracy and productivity

Agilent offers the most comprehensive range of optimized wide-pore, reversed-phase BioHPLC columns, all backed by technical support experts and application chemists around the globe. The family includes 1.8, 3.5, and 5 µm totally porous particles for pressures from 400 to 1200 bar; 3.5 and 5 µm Poroshell (superficially porous) particles for UHPLC separations at lower pressure, and polymeric columns for analysis under the most extreme conditions.

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Charged Variants

Ion-exchange columns to characterize charged variants with speed and confidence

Whether your goal is to characterize the next biopharmaceutical or isolate a target protein, Agilent can help you overcome the challenges of isolating and identifying charged variants of proteins. A wide range of pore sizes means you can effectively separate proteins and charged variants of any size.

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Protein Aggregates and Degradants

Size exclusion columns for fast, high-resolution separation for affinity chromatography

Agilent BioHPLC columns for SEC are easy to integrate into your workflow, and are available in a full range of pore sizes and dimensions giving you the choices you need to perfect separations based on your analytes and method parameters.

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Glycans and Glycopeptides

A complete consumables workflow for the preparation and analysis of 2-AB labeled N-linked glycans, sample preparation, standards, and columns

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Special Offers

Glycan mapping resource kit

Includes application briefs, flyer, and a free poster.

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Titer Determination

Affinity columns for rapid determination of mAb concentrations in fermentation broths and purification of small quantities for characterization
  • Large dynamic range for quantitation over a wide range of product concentrations
  • Ideal for clone selection and optimization
  • Agilent Bio-Monolith Protein A with specificity for IgG subclasses 1 and 2
  • Agilent Bio-Monolith Protein G for IgG subclass 3


High-resolution oligonucleotide analysis with long column lifetime

A key challenge in the development and manufacture of oligonucleotide therapeutics is the need for analytical methods to separate and identify impurities. Agilent AdvanceBio Oligonucleotide columns and standards offer reliable high-resolution oligonucleotide analysis with long column lifetime.

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Desalting Cartridges

Agilent AdvanceBio Desalting-RP Cartridges

With new AdvanceBio Desalting-RP cartridges, you can quickly and efficiently remove salt ions online before MS detection. These flexible, cartridge-style columns can be used in two ways:

  • On their own with any LC system to desalt collected fractions
  • On an Agilent 1290 Infinity II 2D-LC system as a second-dimension desalt after a first-dimension affinity, ion exchange, or size exclusion separation

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For Research Use Only. Not for use in diagnostic procedures.