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Endoglycosidases

Part Number:
GK80110

Enzymatic deglycosylation kit for N-linked and simple O-linked glycans. Contains all enzymes and reagents needed to remove all N-linked glycans and simple O-linked glycans (including polysialylated) from glycoproteins. See the product manual for an example using bovine fetuin.

N-glycanase (PNGase F) cleaves all N-linked complex, hybrid or high mannose oligosaccharides unless α(1,3) core fucosylated (asparagine must be peptide-bonded at both termini).

There is no such universal endoglycosidase for O-glycans. These have to be trimmed down to Gal-GalNAc-O-S/T before O-glycanase (GK80090) can remove the Gal-GalNAc structure. Modification of this core with, e.g., sialic acid will block O-glycanase.

Sialidase A (GK80040) is included in the kit and can remove terminal sialic acid from sialylated T antigen/Core 1 (Sia-Gal-GalNAc-O-S/T) to reveal core 1 Gal-GalNAc. The GK80115 extender kit (β(1,4)Galactosidase and β-N-Acetylglucosaminidase), available separately, can assist with removal of e.g. core 2 structures (GlcNAc-Gal-GalNAc-S/T). For more extended and complex O-glycans structures these enzymes will not be sufficient to reveal Gal-GalNAc. For more on O-GalNAc glycan core structures, please see references

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List Price:
$481.00

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Specifications
Application
  • Epitope and binding site analysis
Enzyme Applications
  • Control glycoprotein provided
  • Deglycosylates up to 2 mg of glycoprotein
  • Native and denaturing protocols
  • No degradation of protein
  • Removes O-linked sugars containing polysialic acid
Literature
Data Sheets

Agilent Glycopro Enzymatic Deglycosylation Kit

Agilent Glycopro Enzymatic Deglycosylation Kit contains all enzymes and reagents needed to completely remove all N-linked and simple O-lined carbohydrates from glycoproteins.

  • Data Sheets
  • English
  • 30-Jul-2019
  • 313.59 Kb
  • PDF
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Support
Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 093, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 093, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 178.14 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 081, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 081, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 177.94 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 080, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 080, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 177.93 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans, DG26 095, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans, DG26 095, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 199.90 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 086, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 086, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 177.93 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 091, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 091, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 177.97 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans, 113 001, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans, 113 001, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 188.91 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 089, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 089, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 177.96 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 094, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 094, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 171.91 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans, 113 002, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked & Simple O-Linked Glycans, 113 002, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 197.36 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 079, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 079, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 178.24 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 084, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 084, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 178.23 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 083, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 083, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 178.15 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 088, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 088, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 177.81 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 082, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 082, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 177.93 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 085, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 085, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 178.32 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 087, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 087, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 178.07 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 090, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 090, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 177.96 Kb
  • PDF

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 092, Certificate of Analysis

GK80110, GlycoPro Enzymatic Deglycosylation Kit for N-Linked &Simple O-Linked Glycans, DG26 092, Certificate of Analysis

  • Certificate of Analysis
  • English
  • 13-Jul-2019
  • 177.92 Kb
  • PDF

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