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AdvanceBio Gly-X 2-AB Express

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AdvanceBio Gly-X N-glycan prep with 2-AB Express kit, 96-ct  (formerly ProZyme). Complete kit. utilizes a novel in-solution enzymatic protein deglycosylation followed by Express labeling of released N-glycans with 2-AB dye. With deglycosylation carried out in solution, the method is simple, rapid, and suitable for automation. After a simple cleanup step, the 2-AB-labeled glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS and other methods. Includes GX96-100 Gly-X deglycosylation module (96-ct), GX96-401 Gly-X 2-AB Express labeling module (96-ct), GX96-402 Gly-X 2-AB Express cleanup module (96-ct).

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  • 5-minute N-Glycanase (PNGase F) digestion provides unbiased N-glycan release
  • Flexible, high-throughput format
    Process 1 to 96 samples
  • Optimized cleanup removes excess free dye, protein, and other interfering compounds.
  • Reductive amination 2-AB labeling chemistry without dry down
  • 96
Deglycosylation Efficiency
  • > 99 %
Equipment & Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)
  • DI water
  • Thermocycler (Corning THERM-1001, 110V, THERM-1000, 230V)
  • Vacuum manifold (Millipore MSVMHTS00-HTS)
  • Vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB starter kit - included free of charge with first Gly-X order, please contact us for details)
  • Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522)
Kit Configuration
  • Sample Prep Kit
  • 2-AB
Prep time (approx)
  • ~2.5 hours
Preservation of Sialylated Species
  • > 99 %
Protein Load Range
  • 1-40 µg
  • > 80 %
  • At least 1 month at -20°C, 5 days at 4–8°C
  • LC & LC/MS
  • 41115711
Kit Contents
Part Number Product Description Quantity
GX96-100 AdvanceBio Gly-X deglycosylation module, 96-ct (formerly ProZyme) 1
GX96-401 AdvanceBio Gly-X 2-AB Express labeling module, 96-ct (formerly ProZyme) 1
GX96-402 AdvanceBio Gly-X 2-AB Express cleanup module, 96-ct (formerly ProZyme) 1

Rapid N-Glycan Sample Prep Workflows for LC and Capillary Electrophoresis Platforms

Rapid N-Glycan Sample Prep Workflows for LC and Capillary Electrophoresis Platforms

Development of a Rapid 2-AB Sample Preparation Workflow for N-Glycans

The characterization of N-glycans is essential to the development of biotherapeutics.

Streamlined Workflows for N-Glycan Analysis of Biotherapeutics with LC/FLD/MS

Streamlined workflows for N-Glycan analysis of biotherapeutics using Agilent AdvanceBio Gly-X InstantPC and 2-AB Express sample preparation with LC/FLD/MS

Preparation of Released N-Glycan Samples from Monoclonal Antibodies Using Agilent AdvanceBio Gly-X 2-AB Express for LC-Fluorescence Analysis

A method using the Agilent AdvanceBio Gly-X 2-AB Express kit and 2-AB labeling for the release of glycans with LC/FLD analysis.

User Manuals

Agilent AdvanceBio Gly-X N-Glycan Prep with 2-AB Express Kit, 96-ct

The Agilent Gly-X N-Glycan Rapid Release and Labeling with 2-AB Express kit uses a novel in-solution enzymatic protein deglycosylation followed by fast labeling of released N-glycans with 2-AB dye.