GKE-5016A
Please login to add product to your favorites.

Endoglycosidases

Part Number:
GKE-5016A

AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL (formerly ProZyme). A recombinant form of PNGase F (100 mU, EDTA free), releases intact N-glycans by cleaving between the innermost GlcNAc and Asn.  Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications).

Item successfully added to your list

Subscribe to this item in cart or checkout

More lab efficiency with your auto delivery schedule, modify and cancel it at any time. Simply select subscription delivery frequency in the cart or checkout, and submit your order. How does it work?

List Price:
$304.00

Please enter valid quantity

Specifications
Concentration
  • 2.5 U/mL
Enzyme Formulation
  • 20 mM Tris-HCl, 50 mM NaCl, (pH 7.5)
Enzyme Source
  • Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity
  • ≥10 units/mg
Enzyme Specificity
  • Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight
  • ~35,000 Daltons
Unit
  • 100 mU
Volume
  • 40 µL
pH Optimum
  • 8.6
pH Range
  • 7.5-9.5
Literature
Data Sheets

Agilent AdvanceBio N-Glycanase

Agilent AdvanceBio N-Glycanase for the release of intact N-linked glycans from glycopeptides and glycoproteins and structure-function studies of N-glycosylated glycoproteins.

Support
Safety Data Sheets

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), >2.5 U/mL

Material Safety Data Sheet

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), >2.5 U/mL

Material Safety Data Sheet

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), >2.5 U/mL

Material Safety Data Sheet