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BL21 Competent Cells

Part Number:

BL21-Gold (DE3)pLysS Competent Cells. For both toxic and non-toxic proteins, pLysS codes for T7 lysozome, a T7 RNA polymerase inhibitor. Use with Variflex or Affinity pCAL vectors. Efficiency is ≥1 x 108 cfu/ µg of supercoiled DNA.

For Research Use Only. Not for use in diagnostic procedures.
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Cell Strain
  • BL21 (DE3)pLysS
Cell Type - Production Method
  • Chemically Competent
Competent Cell Suitable For
  • High-level protein expression and easy induction with IPTG and T&
Difficult Protein Expression Type
  • Toxic Proteins
Generates Unmethylated DNA
  • No
  • E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA Hte [pLysS Camr]
  • Chloramphenicol
  • Tetracycline
Transformation Efficiency - Quantitative
  • 1 x 10^8
  • 10 x 0.1 mL