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AdvanceBio Gly-X Technology

Part Number:
GX96-IQ

AdvanceBio Gly-X N-glycan prep with InstantQ kit, 96-ct (formerly ProZyme). Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantQ dye. After a simple cleanup step, the glycan samples are ready for analysis on the Gly-Q glycan analysis system. With deglycosylation and labeling carried out in solution, the method is simple, rapid and suitable for automation. Includes GX96-300 Gly-X deglycosylation module for InstantQ, GX96-301 Gly-X InstantQ labeling module, GX96-302 Gly-X InstantQ cleanup module, GKSQ-505 Gly-Q alignment standards set (GKSQ-500 & GKSQ-503), GQ103 Gly-Q cartridge module.

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Specifications
Application
  • Flexible, high-throughput format: process 1 to 96 samples
Count
  • 96
Deglycosylation Efficiency
  • > 99 %
Equipment and Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)
  • Formic acid, MS-grade (Fisher A117-50 recommended)
  • Thermocycler (Corning THERM-1001, 110V. THERM-1000, 230V)
  • Vacuum manifold (Millipore MSVMHTS00-HTS)
  • Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220 V. Welch WOB-L pump 2522)
Instrument Compatibility
  • Gly-Q Glycan Analysis System
Kit Configuration
  • Sample Prep Kit
Label
  • InstantQ
Precision (well-to-well, day-to-day)
  • <5% CV
Prep time (approx)
  • ~1 hour
Preservation of Sialylated Species
  • > 95 %
Protein Load Range
  • 1-40 µg
Recovery
  • > 85 %
Stability
  • up to 18 hrs on RT autosampler, and -20°C for up to 30 days in water
Technique
  • CE
Literature
Applications

High-Throughput CE N-Glycan Analysis for Clone Screening

Rapid N-glycan preparation and screening of biosimilar candidates by LC/FLD/MS and CE.

Rapid N-Glycan Sample Prep Workflows for LC and Capillary Electrophoresis Platforms

Rapid N-Glycan Sample Prep Workflows for LC and Capillary Electrophoresis Platforms

Automation of Gly-X N-Glycan Sample Prep with InstantPC and InstantQ Dyes

Automation of Gly-X N-Glycan Sample Prep with InstantPC and InstantQ Dyes

Qly-Q Integrated Solution for High-Throughput Glycoanalysis Using Rapid CE

Qly-Q Integrated Solution for High-Throughput Glycoanalysis Using Rapid CE

Support
User Manuals

Agilent AdvanceBio Gly-X N-Glycan Prep with InstantQ Kit, 96-ct

The Agilent Gly-X N-Glycan Rapid Release and Labeling with InstantQ kit utilizes a novel in-solution enzymatic protein deglycosylation with N-Glycanase followed by fast labeling of released N-glycans with InstantQ dye.