GX96-IPC
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AdvanceBio Gly-X Technology

Part Number:
GX96-IPC

AdvanceBio Gly-X N-glycan prep with InstantPC kit, 96-ct (formerly ProZyme). Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantPC dye. After a simple cleanup step, the glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS and other methods. With deglycosylation and labeling carried out in solution, the method is simple, rapid, and suitable for automation. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, that enables a single labeling method to be deployed across different glycan analysis workflows. Sample preparation time: ~1 hour. Includes GX96-100 Gly-X deglycosylation module, GX96-101 Gly-X InstantPC labeling module, GX96-102 Gly-X InstantPC cleanup module.

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Specifications
Application
  • Flexible, high-throughput format: process 1 to 96 samples
Count
  • 96
Equipment and Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks
  • Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)
Kit Configuration
  • Sample Prep Kit
Label
  • InstantPC
Precision (well-to-well, day-to-day)
  • <5% CV
Prep time (approx)
  • ~1 hour
Preservation of Sialylated Species
  • > 95 %
Protein Load Range
  • 1-40 µg
Recovery
  • > 95 %
Stability
  • Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF
Technique
  • LC & LC/MS
Kit Contents
Part Number Product Description Quantity
GX96-100 AdvanceBio Gly-X deglycosylation module, 96-ct (formerly ProZyme) 1
GX96-101 AdvanceBio Gly-X InstantPC labeling module, 96-ct (formerly ProZyme) 1
GX96-102 AdvanceBio Gly-X InstantPC cleanup module, 96-ct (formerly ProZyme) 1
Literature
Applications

High-Throughput CE N-Glycan Analysis for Clone Screening

Rapid N-glycan preparation and screening of biosimilar candidates by LC/FLD/MS and CE.

Rapid N-Glycan Sample Prep Workflows for LC and Capillary Electrophoresis Platforms

Rapid N-Glycan Sample Prep Workflows for LC and Capillary Electrophoresis Platforms

Automation of Gly-X N-Glycan Sample Prep with InstantPC and InstantQ Dyes

Automation of Gly-X N-Glycan Sample Prep with InstantPC and InstantQ Dyes

High-Throughput Analysis of N-Glycans Released from Biosimilar Glycoproteins

Comparison of Common Fluorescent Labels for LC Analysis of Released N-Linked Glycans

Streamlined Workflows for N-Glycan Analysis of Biotherapeutics with LC/FLD/MS

Streamlined Workflows for N-Glycan Analysis of Biotherapeutics Using Agilent AdvanceBio Gly-X InstantPC and 2-AB Express Sample Preparation with LC/FLD/MS

Support
User Manuals

Agilent AdvanceBio Gly-X InstantPC Quick Start Guide

Prepare N-glycan samples in 1 hour using Agilent AdvanceBio Gly-X InstantPC with this simple step by step process.

Agilent AdvanceBio Gly-X N-Glycan Prep with InstantPC Kit, 96-ct

The Agilent Gly-X N-Glycan Rapid Release and Labeling with InstantPC kit utilizes a novel in-solution enzymatic protein deglycosylation with N-Glycanase followed by fast labeling of released N-glycans with InstantPC dye.