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AdvanceBio Gly-X Technology

Part Number:
GX96-APTS

AdvanceBio Gly-X N-glycan prep with APTS Express kit, 96-ct (formerly ProZyme). Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by Express labeling of released N-glycans with 8-aminopyrene-1,3,6-trisulfonic acid (APTS). With deglycosylation carried out in solution, the method is simple, rapid and suitable for automation. After a simple cleanup step, the APTS-labeled glycan samples are ready for analysis by CE, CE/MS, and other methods. Includes GX96-100 Gly-X deglycosylation module (96-ct), GX96-601 Gly-X APTS Express labeling module (96-ct), GX96-602 Gly-X APTS Express cleanup module (96-ct).

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Specifications
Equipment and Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C
  • Thermocycler (Corning THERM-1001, 110 V. THERM-1000, 230 V)
  • Vacuum manifold (Millipore MSVMHTS00-HTS)
  • Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220V. Welch WOB-L pump 2522)
  • Vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB/APTS starter kit is included free of charge with first Gly-X order, please contact us for details)
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended
  • DI Water
Protein Load Range
  • 1-40 µg
Label
  • APTS
Prep time (approx)
  • ~2.5 hours
Application
    96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C
  • Thermocycler (Corning THERM-1001, 110 V. THERM-1000, 230 V)
  • Vacuum manifold (Millipore MSVMHTS00-HTS)
  • Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220V. Welch WOB-L pump 2522)
  • Vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB/APTS starter kit is included free of charge with first Gly-X order, please contact us for details)
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended
  • DI Water
  • Flexible, high-throughput format: process 1 to 96 samples
Count
  • 96
Technique
  • CE
Kit Configuration
  • Sample Prep Kit
Kit Contents
Part Number Product Description Quantity
GX96-601 AdvanceBio Gly-X APTS Express labeling module, 96-ct (formerly ProZyme) 1
GX96-602 AdvanceBio Gly-X APTS Express cleanup module, 96-ct (formerly ProZyme) 1
GX96-100 AdvanceBio Gly-X deglycosylation module, 96-ct (formerly ProZyme) 1
Resources

Development of a Rapid APTS Sample Preparation Workflow for N-Glycans

Comparison of the fluorescence and MS response of Agilent InstantDyes using the Agilent Glykoprep Rapid N-glycan preparation system.

Agilent Gly-X N-Glycan Rapid Release and Labeling with APTS Express Kit

The Agilent Gly-X N-Glycan Rapid Release and Labeling with APTS Express Kit utilizes a novel in-solution enzymatic protein deglycosylation with N-Glycanase followed by fast labeling of released N-glycans with APTS dye.

AdvanceBio Gly-X N-Glycan Prep with APTS Kit, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X Reductant Solution for APTS, 134μl, Gly-X APTS Solution, 336μlm, Gly-X APTS Catalyst, 370μl, Gly-X APTS Finishing Reagent, 230μl, Waste Tray, Gly-X APTS Cleanup Solution

AdvanceBio Gly-X N-Glycan Prep with APTS Kit, 96-ct

Gly-X-Glycanase, 1 mg/mL, 120 μl, Gly-X Digestion Buffer, 240 μl, Gly-X Denaturant, 240 μl, Gly-X Reductant Solution for APTS, 134μl, Gly-X APTS Solution, 336μlm, Gly-X APTS Catalyst, 370μl, Gly-X APTS Finishing Reagent, 230μl, Waste Tray, Gly-X APTS Cleanup Solution