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AdvanceBio Gly-X Technology

Part Number:
GX24-IQ

AdvanceBio Gly-X N-glycan prep with InstantQ Kit, 24-ct (formerly ProZyme). Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantQ dye. InstantQ dye is specifically designed for glycan separation and detection using the Gly-Q instrument and a Gly-Q InstantQ cartridge. After a simple cleanup step, the glycan samples are ready for analysis on the Gly-Q glycan analysis system. With deglycosylation and labeling carried out in solution, the method is simple, rapid, and suitable for automation. Includes GX24-300 Gly-X deglycosylation module for InstantQ, GX24-301 Gly-X InstantQ labeling module, GX96-302 Gly-X InstantQ cleanup module, GKSQ-505 Gly-Q alignment standards set (GKSQ-500 and GKSQ-503), GQ103 Gly-Q cartridge module.

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Specifications
Application
  • Flexible, high-throughput format: process 1 to 24 samples
Count
  • 24
Deglycosylation Efficiency
  • > 99 %
Equipment and Reagents Provided by User
  • Flexible, high-throughput format: process 1 to 24 samples
Instrument Compatibility
  • Gly-Q Glycan Analysis System
Kit Configuration
  • Sample Prep Kit
Label
  • InstantQ
Precision (well-to-well, day-to-day)
  • <3% CV
Prep time (approx)
  • 30 min (12 samples), 90 min (96 samples)
Preservation of Sialylated Species
  • > 95 %
Protein Load Range
  • 1-40 µg
Recovery
  • > 85 %
Stability
  • up to 18 hrs on RT autosampler, and -20°C for up to 30 days in water
Technique
  • CE
Literature
Applications

Rapid N-Glycan Sample Prep Workflows for LC and Capillary Electrophoresis Platforms

Rapid N-Glycan Sample Prep Workflows for LC and Capillary Electrophoresis Platforms

Automation of Gly-X N-Glycan Sample Prep with InstantPC and InstantQ Dyes

Automation of Gly-X N-Glycan Sample Prep with InstantPC and InstantQ Dyes

Qly-Q Integrated Solution for High-Throughput Glycoanalysis Using Rapid CE

Qly-Q Integrated Solution for High-Throughput Glycoanalysis Using Rapid CE

High-Throughput CE N-Glycan Analysis for Clone Screening

Rapid N-glycan preparation and screening of biosimilar candidates by LC/FLD/MS and CE.