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Part Number:
GK80090

Endoglycosidases

AdvanceBio O-Glycanase (formerly ProZyme). Releases unsubstituted Ser/Thr-linked GalGalNAc from proteins and can be used with additional enzymes to remove more complex O-linked structures. Also known as Endo-O-Glycosidase, Endo-α N-acetylgalactosaminidase, O-Glycosidase. Includes 5X reaction buffer A [250 mM sodium phosphate pH 5.0]

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List Price:
$256.00

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Specifications
Volume
  • 40 µL
Enzyme Applications
  • Detection of O-glycosylation in proteins
  • Epitope and binding site analysis
  • Bioactivity in O-glycans
  • Analysis of biosynthesis of glycoproteins
  • Structural analysis of O-glycan
  • Glycoprotein deglycosylation
Concentration
  • ≥1.25 U/mL
pH Optimum
  • 5.5
Unit
  • 50 mU
Molecular Weight
  • ~180 kD
Enzyme Specific Activity
  • ≥12 units/mg
pH Range
  • 5-7
Application
  • Detection of O-glycosylation in proteins
Enzyme Specificity
  • The enzyme is highly specific, liberating Galß1-3GalNAc from serine or threonine residues alone or as part of glycopeptides or glycoproteins. There is no apparent preference for serine over threonine. Substitution of the disaccharide core with sialic acid, fucose, N-acetylglucosamine or N-acetylgalactosamine residues, prevents cleavage of the Galß1-3GalNAc-Ser/Thr linkage. Absence of the galactose residue or the acetamido group on the innermost N-acetylgalactosamine residue also prevents cleavage of the Galß1-3GalNAc-Ser/Thr linkage. O-Glycanase is included in the Agilent Enzymatic Deglycosylation kit for N-linked and simple O-linked glycans (GK80110), which may be used in conjunction with our the Agilent Extender kit for complex O-linked glycans (GK80115).
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of Galß1-3GalNAca1-p-nitrophenol per minute at 37°C, pH 5.5.
Enzyme Formulation
  • 20 mM Tris HCl pH 7.5 containing 25 mM NaCl.
Enzyme Source
  • Recombinant gene from Streptococcus pneumoniae, expressed in E.coli.
Resources

GK80090, O-GLYCANASE (recombinant from Streptococcus pneumoniae expressed in E. coli), 155 001a-1, Certificate of Analysis

GK80090, O-GLYCANASE (recombinant from Streptococcus pneumoniae expressed in E. coli), 155 001a-1, Certificate of Analysis

GK80090, O-GLYCANASE (recombinant from Streptococcus pneumoniaeexpressed in E. coli), DG53 046c, Certificate of Analysis

GK80090, O-GLYCANASE (recombinant from Streptococcus pneumoniaeexpressed in E. coli), DG53 046c, Certificate of Analysis

GK80090, O-GLYCANASE (recombinant from Streptococcus pneumoniaeexpressed in E. coli), DG53 046a, Certificate of Analysis

GK80090, O-GLYCANASE (recombinant from Streptococcus pneumoniaeexpressed in E. coli), DG53 046a, Certificate of Analysis

GK80090 O-Glycanase user manual

User manual for GK80090 O-Glycanase

GK80090, O-GLYCANASE (recombinant from Streptococcus pneumoniaeexpressed in E. coli), DG53 047a, Certificate of Analysis

GK80090, O-GLYCANASE (recombinant from Streptococcus pneumoniaeexpressed in E. coli), DG53 047a, Certificate of Analysis

GK80090, O-GLYCANASE (recombinant from Streptococcus pneumoniaeexpressed in E. coli), DG53 046b, Certificate of Analysis

GK80090, O-GLYCANASE (recombinant from Streptococcus pneumoniaeexpressed in E. coli), DG53 046b, Certificate of Analysis

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml

AdvanceBio O-Glycanase, 5x Reaction Buffer A

AdvanceBio O-Glycanase, 50 mU, 5x Reaction Buffer A, 1 ml