GKX-5014
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Exoglycosidases

Part Number:
GKX-5014

β(1-4)-Galactosidase (streptococcus pneumoniae). Enzyme releases nonreducing terminal β(1-4)-linked galactose from oligosaccharides and glycoproteins. This specificity is only evident at enzyme concentrations < 100mU/mL. At higher concentrations, hydrolysis of β(1-3)-linked galactose occurs. Includes 5x reaction buffer B (250 mM sodium phosphate, pH 6.0).

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Specifications
Concentration
  • 2 U/mL
Enzyme Applications
  • Due to its high selectivity the enzyme is an extremely useful reagent for the identification of nonreducing terminal ß(1-4)-linked galactose residues. As such the enzyme has been extensively used for detailed structural analysis with broader specificity bovine testes ß-galactosidase (GKX-5013) or jack bean ß-galactosidase (GKX-5012).
Enzyme Formulation
  • 20 mM Tris-HCl, 25 mM NaCl (pH 7.5)
Enzyme Source
  • Streptococcus pneumoniae.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to hydrolyze 1 µmole pNP-ß-D-galactopyranoside per min at pH 6.0 and 37°C.
Molecular Weight
  • 220-247 kDa
Unit
  • 200 mU
Volume
  • 100 µL
pH Optimum
  • 6
Support
Safety Data Sheets

β(1,4)-Galactosidase (Streptococcus pneumoniae)

β(1,4)-Galactosidase (Streptococcus pneumoniae), 100 μl, 5x Reaction Buffer B, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 461.66 Kb
  • PDF

β(1,4)-Galactosidase (Streptococcus pneumoniae)

β(1,4)-Galactosidase (Streptococcus pneumoniae), 100 μl, 5x Reaction Buffer B, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 417.27 Kb
  • PDF

β(1,4)-Galactosidase (Streptococcus pneumoniae)

β(1,4)-Galactosidase (Streptococcus pneumoniae), 100 μl, 5x Reaction Buffer B, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 452.85 Kb
  • PDF

β(1,4)-Galactosidase (Streptococcus pneumoniae)

β(1,4)-Galactosidase (Streptococcus pneumoniae), 100 μl, 5x Reaction Buffer B, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 520.93 Kb
  • PDF

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