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Endoglycosidases

Part Number:
GKE-5011A

AdvanceBio PNGase A (almond), 2 mU (formerly Prozyme). Enzyme cleaves N-linked glycans from glycopeptides, including glycans with α(1,3)-linked core fucose as seen in insects and plants. Incudes reaction buffer: 50 mM citrate-phosphate buffer, pH 5.0, 0.02% sodium azide.

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List Price:
$367.00

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Specifications
Enzyme Formulation
  • Lyophilized powder containing citrate-phosphate buffer, pH 5.0.
Enzyme Source
  • Almond. Enzyme also knows as Peptide N-Glycosidase A, Glycopeptidase from almonds, Almond glycoamidase, Peptide-N(4)-(N-acetyl-β glucosaminyl) asparagine amidase.
Enzyme Specific Activity
  • > 5 units/mg
Enzyme Specificity
  • PNGase A from almonds cleaves N-glycan chains linked to Asparagine from glycopeptides. The enzyme hydrolyzes an N(4)-(acetyl-ß D-glucosaminyl) asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-ß D-glucosaminylamine and a peptide containing an aspartate residue. The enzyme cleaves N-glycans with or without a(1,3)-linked core fucose residues present in insect and plant glycoproteins. This differs from PNGase F, which cannot remove N-glycans with a(1,3)-linked core fucose. PNGase A requires glycopeptides as substrates. Glycopeptides can be obtained by digesting the glycoprotein with trypsin [1,2] or pepsin. Does not act on (GlcNAc)-Asn, because it requires the presence of more than two amino acid residues in the substrate.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to hydrolyze 1 µmol ovalbumin glycopeptide per min at pH 5.0 and 37°C.
Molecular Weight
  • 54.2 and 21.2 kDa
Unit
  • 2 mU
pH Optimum
  • 5
pH Range
  • 4-6
Support
Safety Data Sheets

AdvanceBio PNGase A (almond)

AdvanceBio PNGase A (almond), 2 mU; AdvanceBio PNGase A (almond), 10 mU

AdvanceBio PNGase A (almond)

AdvanceBio PNGase A (almond), 2 mU; AdvanceBio PNGase A (almond), 10 mU

AdvanceBio PNGase A (almond), 2 mU

AdvanceBio PNGase A (almond), 2 mU, Reaction Buffer for GKE-5011, 2 ml

AdvanceBio PNGase A (almond), 2 mU

AdvanceBio PNGase A (almond), 2 mU, Reaction Buffer for GKE-5011, 2 ml

AdvanceBio PNGase A (almond), 2 mU

AdvanceBio PNGase A (almond), 2 mU, Reaction Buffer for GKE-5011, 2 ml

AdvanceBio PNGase A (almond)

AdvanceBio PNGase A (almond), 2 mU; AdvanceBio PNGase A (almond), 10 mU

AdvanceBio PNGase A (almond)

AdvanceBio PNGase A (almond), 2 mU; AdvanceBio PNGase A (almond), 10 mU

AdvanceBio PNGase A (almond), 2 mU

AdvanceBio PNGase A (almond), 2 mU, Reaction Buffer for GKE-5011, 2 ml

Certificate of Analysis

GKE-5011A, GLYKO PNGASE A (Peptide N-Glycosidase from Almond), DH59 007, Certificate of Analysis

GKE-5011A, GLYKO PNGASE A (Peptide N-Glycosidase from Almond), DH59 007, Certificate of Analysis