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T3 RNA Polymerase. High sequence specifity for T3 promoters

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Specifications
Concentration
  • 50 U/µL
Incubation Time
  • Incubate 30 minutes at 37 °C
Reaction Conditions
  • 40mM Tris-HCl (pH 8.0), 8 mM MgCl2, 50 mM NaCl, 2 mM spermidine, 30 mM DTT, 400 µM of each rNTP, 1 µg DNA template and 10 units enzyme in a 25 µL volume. Incubate 30 minutes at 37°C
Reactions per Kit
  • 5000 units
Storage Buffer
  • 20 mM KPO4 (pH 7.7), 100 mM NaCl, 10 mM DTT, 1 mM EDTA, and 50 % glycerol (v/v)
Unit Definition
  • One unit will catalize the incorporation of 1.0 nmole of nucleoside triphosphate into an acid insoluble form in 60 minutes at 37°C