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BL21-CodonPlus Competent Cells

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BL21-CodonPlus (DE3)-RIPL Competent Cells. These cells contain extra copies of rare E. coli argU, ileY, leuW, proL tRNA genes which corrects for codon bias and dramatically improves expression of sequences from other organisms.

For Research Use Only. Not for use in diagnostic procedures.
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Cell Strain
  • BL21-CodonPlus (DE3)-RIPL
Cell Type - Production Method
  • Chemically Competent
Competent Cell Suitable For
  • Improved codon bias performance and IPTG induction
Difficult Protein Expression Type
  • Codon Bias Problems
Extra Copies of tRNA Genes
  • argU
  • ileY
  • leuW
  • proL
Generates Unmethylated DNA
  • No
  • E. coli B F– ompT hsdS(rB – mB – ) dcm+ Tetr gal l(DE3) endA Hte [argU proL Camr ] [argU ileY leuW Step/Specr ]
Host System
  • Bacterial
  • Insect
  • Mammalian
  • Plant
  • Yeast
  • Chloramphenicol
  • Streptomycin
Transformation Efficiency - Quantitative
  • 1 x 10^7
  • 10 x 0.1 mL