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BL21-CodonPlus Competent Cells

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BL21-CodonPlus (DE3)-RP-X Competent cells. Encodes T7 RNA polymerase under control of the lacUV5 promoter for easy protein expression plus methionine label, Use with pET or pCAL vectors, Used for labeling cells 1 x 107 cfu/µg of supercoiled DNA.

For Research Use Only. Not for use in diagnostic procedures.
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Cell Strain
  • BL21-CodonPlus (DE3)-RP-X
Cell Type - Production Method
  • Chemically Competent
Competent Cell Suitable For
  • Improved codon bias performance and high expression levels - not for use with toxic proteins
Difficult Protein Expression Type
  • Codon Bias Problems
Extra Copies of tRNA Genes
  • argU
  • proL
Generates Unmethylated DNA
  • No
  • E. coli B F– ompT hsdS(rB – mB – ) dcm+ Tetr gal l (DE3) endA Hte metA::Tn5(Kanr ) [argU proL Camr ]
Host System
  • Bacterial
  • Insect
  • Mammalian
  • Plant
  • Yeast
  • Tetracycline
Transformation Efficiency - Quantitative
  • 1 x 10^7
  • 10 x 0.1 mL