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GlykoPrep Technology

Part Number:
GP24NG-PC

GlykoPrep Rapid N-glycan Prep with InstantPC, 24-ct. Complete kit, N-glycan sample preparation cartridge-based workflow with rapid digestion, instant labeling, and cleanup of excess InstantPC label. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, which enables a single labeling method to be deployed across different glycan analysis workflows. Includes GS24-RX digestion module; GS24-PC InstantPC labeling module; GS24-CU cleanup module.

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Specifications
Application
  • Flexible, format: process 1 to 24 samples per run
Count
  • 24
Equipment & Reagents Provided by User
  • Acetonitrile (ACN, 100%, HPLC-grade)
  • AssayMAP Labware (AM200)
  • Centrifuge (capable of 50 - 1000 x g) and deep microplate rotor with clearance of minimum 44 mm height
  • Formic acid (HPLC-grade)
  • Heater and incubation blocks, capable of 50–100°C, available from Agilent (GS150)
Kit Configuration
  • Sample Prep Kit
Label
  • InstantPC
Prep time (approx)
  • 3 hours
Technique
  • LC & LC/MS
Kit Contents
Part Number Product Description Quantity
GS24-CU GlykoPrep cleanup module, 24-ct 1
GS24-PC Agilent GlykoPrep InstantPC Labeling Module, 24-ct 1
GS24-RX GlykoPrep digestion module, 24-ct 1
Literature
Applications

Comparison of Relative Quantification of Monoclonal Antibody N-glycans Using Fluorescence and MS Detection

Comparison of Relative Quantification of Monoclonal Antibody N-glycans Using Fluorescence and MS Detection

  • Applications
  • English
  • 29-Jun-2016
  • 2.09 Mb
  • PDF

Profiling Glycosylation of Monoclonal Antibodies at Three Levels Using the Agilent 6545XT AdvanceBio LC/Q-TOF

This application note demonstrates three approaches to quantitatively understand the glycosylation present for a given mAb.

  • Applications
  • English
  • 22-Jan-2018
  • 1.20 Mb
  • PDF
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Support
User Manuals

InstantPC Microfuge Method

User manual for Microfuge IPC

  • User Manuals
  • English
  • 04-Aug-2019
  • 403.05 Kb
  • PDF

2-AB Microfuge Method

User manual for 2-AB Microfuge Method

  • User Manuals
  • English
  • 04-Aug-2019
  • 397.33 Kb
  • PDF

GlykoPrep Rapid N-Glycan Preparation with InstantPC™

User manual for GP96NG-PC Rapid N-Glycan Preparation with InstantPC

  • User Manuals
  • English
  • 01-Aug-2019
  • 1.42 Mb
  • PDF

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