DNF-476-0500
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Fragment Analyzer DNA/NGS Kits

Part Number:
DNF-476-0500
RUO

Small Fragment Kit, 500. Fragment Analyzer system kit for the quantitative and qualitative analysis of small dsDNA fragments and smears as well as PCR DNA fragments from 50 to 1500 bp at high concentrations. Includes all the reagents necessary for running 500 samples

For Research Use Only. Not for use in diagnostic procedures.
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List Price:
$1,686.00 /1 Kit

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Specifications
Analysis Time
  • Short array 45 min
Assay Sensitivity
  • Standard
DNA Sizing Range
  • 50 - 1500 bp
Fragment Sensitivity Range
  • 0.1 - 10 ng/µL
Guaranteed Shelf Life
  • Minimum 4 months
Kit Type
  • Quantitative
Quantitative Accuracy
  • ± 25 %
Quantitative Precision
  • 15 % CV
Sample Volume
  • 2 µL
Sizing Accuracy
  • ± 5 %
Sizing Precision
  • 2 % CV
Sizing Resolution
  • 50 - 900 bp: ≤ 5 %
  • 900 - 1500 bp: ≤ 10 %
Smear Sensitivity Range
  • 5 - 100 ng/µL
Uses Per Kit
  • 500
Kit Contents
Part Number Product Description Quantity
DNF-230-0240 Small Fragment Separation Gel, 240mL 1
DNF-300-0008 BF-25 Blank Solution, 8mL 1
DNF-355-0125 5x 930 dsDNA Inlet Buffer, 125mL 1
DNF-362-0003 Small Fragment Diluent Marker, 2.4 mL 5
DNF-363-U100 Small Fragment DNA Ladder, 100uL 1
DNF-475-0050 5x Capillary Conditioning Solution, 50mL 1
DNF-497-0125 0.25x TE Rinse Buffer, 125mL 1
DNF-600-U030 Intercalating Dye, 30 µL 1
Support
User Manuals

Agilent DNF-476 Small Fragment Kit Quick Guide for Fragment Analyzer Systems

This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The Small Fragment kit (p/n DNF-476-0500) is designed for sizing and quantification of dsDNA smears/fragments between 50 bp and 1,500 bp. Example applications include quality control of Next-Generation Sequencing (NGS) libraries or quantitative PCR fragment analysis.

  • User Manuals
  • English
  • 04-Jul-2022
  • 252.43 Kb
  • PDF

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