659750-702
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AdvanceBio Oligonucleotide

Part Number:
659750-702

Agilent AdvanceBio Oligonucleotide, 2.1 x 50 mm, 2.7 µm. Reversed-phase HPLC column designed for high-resolution IP-RP separation of oligos. Scalable chemistry platform supports analytical through prep purification for optimal resolution, yield, purity.

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Specifications
Brand
  • AdvanceBio Oligonucleotide
Hardware
  • SS
Inner Diameter (ID)
  • 2.1 mm
LC Platform
  • Stainless Steel
Length
  • 50 mm
Maximum Temperature
  • 65 °C
Part Number Application
  • Oligos
Part Number Application Domain
  • Biopharma
  • Pharmaceutical
Particle Size
  • 2.7 µm
Particle Type
  • Superficially Porous
Phase
  • HPH-C18
Pore Size
  • 120 Å
Pressure Rating
  • 600 bar
Separation Mode
  • Reversed Phase
Shipping Solvent
  • Acetonitrile/Water
Technique
  • LC & LC/MS
UNSPSC Code
  • 41115711
pH Range
  • 3-11
Literature
Applications

Comprehensive and Integrated Workflow for Oligonucleotide Sequence Confirmation by Agilent High-Resolution LC/Q-TOF

This application note demonstrates an oligonucleotide sequence confirmation workflow using Agilent BioConfirm software with an Agilent 1290 Infinity II LC and Agilent 6545XT AdvanceBio LC/Q-TOF.

  • Applications
  • English
  • 13-Jul-2022
  • 2.40 Mb
  • PDF

Rapid Analysis of mRNA 5' Capping with High Resolution LC/MS

Rapid mRNA capping analysis with a combined sample preparation and analysis time of only 75 minutes using an Agilent 6545XT AdvanceBio LC/Q-TOF.

  • Applications
  • English
  • 21-Sep-2021
  • 1.33 Mb
  • PDF

Fast and High-Resolution Reversed-Phase Separation of Synthetic Oligonucleotides

AdvanceBio Oligonucleotide with UV and MS eluents is ideal for oligonucleotides, with exceptional resolution, superior lifetime, compatible with 600 and 1200 bar.

  • Applications
  • English
  • 25-Jun-2015
  • 330.43 Kb
  • PDF

Evaluation of Different Ion Pairing Reagents for LC/UV and LC/MS Analysis of Oligonucleotides

This application note shows that there are numerous variables available for optimization of the analytical conditions for oligonucleotides. The analyses were carried out on an Agilent 1290 Infinity II LC System with UV detection by DAD and an Agilent 6530 LC/Q-TOF.

  • Applications
  • English
  • 04-Jan-2021
  • 806.12 Kb
  • PDF
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