TMB S2, ELISA HRP Substrate, 1L, Ready-to-use. TMB S2 is a ready-to-use reagent containing 3,3',5,5'-tetramethylbenzidine (TMB), a chromogenic substrate for horseradish peroxidase (HRP) for highly sensitive detection. During the enzymatic degradation of hydrogen peroxide by HRP, TMB forms a water-soluble blue byproduct that can be measured spectrophotometrically at 650 nm. Acidification with a stop solution of sulphuric acid (H2SO4) renders a yellow color with absorption peak at 450 nm.
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