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AdvanceBio Gly-X Technology

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AdvanceBio Gly-X deglycosylation and InstantPC labeling module set, 96-ct (formerly ProZyme). Utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantPC dye. With deglycosylation and labeling carried out in solution, the method is simple, rapid, and suitable for automation. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, which enables a single labeling method to be deployed across different glycan analysis workflows. Includes GX96-100 Gly-X deglycosylation module,
GX96-101 Gly-X InstantPC labeling module. For cleanup use GX96-102 Gly-X InstantPC cleanup module.

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  • Flexible, high-throughput format: process 1 to 96 samples
  • 96
Equipment & Reagents Provided by User
  • Thermocycler (Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks
Kit Configuration
  • Sample Prep Module Set
  • InstantPC
Precision (well-to-well, day-to-day)
  • <5% CV
Prep time (approx)
  • 42 min (16 samples), 99 min (96 samples)
Preservation of Sialylated Species
  • > 95 %
Protein Load Range
  • 1-40 µg
  • > 95 %
  • Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF
  • LC & LC/MS
Kit Contents
Part Number Product Description Quantity
GX96-100 AdvanceBio Gly-X deglycosylation module, 96-ct (formerly ProZyme) 1
GX96-101 AdvanceBio Gly-X InstantPC labeling module, 96-ct (formerly ProZyme) 1