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AdvanceBio Gly-X N-glycan prep with InstantQ Kit, 24-ct (formerly ProZyme). Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantQ dye. InstantQ dye is specifically designed for glycan separation and detection using the Gly-Q instrument and a Gly-Q InstantQ cartridge. After a simple cleanup step, the glycan samples are ready for analysis on the Gly-Q glycan analysis system. With deglycosylation and labeling carried out in solution, the method is simple, rapid, and suitable for automation. Includes GX24-300 Gly-X deglycosylation module for InstantQ, GX24-301 Gly-X InstantQ labeling module, GX96-302 Gly-X InstantQ cleanup module, GKSQ-505 Gly-Q alignment standards set (GKSQ-500 and GKSQ-503), GQ103 Gly-Q cartridge module.

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Specifications
Application
  • Flexible, high-throughput format: process 1 to 24 samples
Count
  • 24
Deglycosylation Efficiency
  • > 99 %
Equipment & Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)
  • Formic acid, MS-grade (Fisher A117-50 recommended)
  • THERM-1000, 230V)
  • Thermocycler (Corning THERM-1001, 110V
  • Vacuum manifold (Millipore MSVMHTS00-HTS)
  • Vacuum pump (Millipore WP6211560, 110 V
  • WP6122050, 220V
  • Welch WOB-L Pump 2522)
Instrument Compatibility
  • Gly-Q Glycan Analysis System
Kit Configuration
  • Sample Prep Kit
Label
  • InstantQ
Precision (well-to-well, day-to-day)
  • <3% CV
Prep time (approx)
  • 30 min (12 samples), 90 min (96 samples)
Preservation of Sialylated Species
  • > 95 %
Protein Load Range
  • 1-40 µg
Recovery
  • > 85 %
Stability
  • Up to 18 hrs on RT autosampler, and -20°C for up to 30 days in water
Technique
  • CE
Literature
Applications

High-Throughput CE N-Glycan Analysis for Clone Screening

Rapid N-glycan preparation and screening of biosimilar candidates by LC/FLD/MS and CE.

  • Applications
  • English
  • 02-Aug-2019
  • 715.67 Kb
  • PDF
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