Part Number:
GKE-5020B

AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), ≥10 U/mL (formerly ProZyme). A recombinant form of PNGase F (400 mU, EDTA free), releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. The same formulation provided in GlykoPrep, ≥ 10 U/mL and EDTA-free. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution

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List Price:

$1,365.00

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Specifications

Concentration	10 U/mL
Enzyme Applications	To obtain efficient deglycosylation of glycoprotein substrates under non-denaturing conditions, it is necessary to use a higher starting concentration of enzyme. N-Glycanase PLUS and ULTRA (EDTA-Free) are supplied at = 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller reaction volumes and shorter reaction times to be explored.
Enzyme Formulation	20 mM Tris-HCl, 50 mM NaCl (pH 7.5, EDTA-free formulation).
Enzyme Source	Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity	≥10 units/mg
Enzyme Specificity	Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight	~35,000 Daltons
Unit	400 mU
Volume	40 µL
pH Optimum	8.6
pH Range	7.5-9.5

Literature

Data Sheets

Agilent AdvanceBio N-Glycanase-ULTRA

Agilent AdvanceBio N-Glycanase ULTRA for the removal of N-glycans from glycoproteins and glycopeptides.

Data Sheets
English
30-Jul-2019
571.71 Kb
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Safety Data Sheets

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL
AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

Safety Data Sheets

English

12-May-2019

198.08 Kb

PDF

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL
AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

Safety Data Sheets

English

12-May-2019

166.62 Kb

PDF

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), >10 U/mL
Material Safety Data Sheet

Safety Data Sheets

English

25-Jan-2022

553.57 Kb

PDF

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL
AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

Safety Data Sheets

English

12-May-2019

69.05 Kb

PDF

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL
AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

Safety Data Sheets

English

12-May-2019

170.32 Kb

PDF

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), >10 U/mL
Material Safety Data Sheet

Safety Data Sheets

English

25-Jan-2022

566.17 Kb

PDF

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), >10 U/mL
Material Safety Data Sheet

Safety Data Sheets

English

25-Jan-2022

551.72 Kb

PDF

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