DNF-473-1000
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Fragment Analyzer DNA/NGS Kits

Part Number:
DNF-473-1000
RUO

NGS Fragment Kit (1-6000bp), 1000. Fragment Analyzer system kit for the quantitative and qualitative analysis of NGS libraries and intermediates from 100 to 6000 bp. Includes reagents necessary for running 1000 samples.

For Research Use Only. Not for use in diagnostic procedures.
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List Price:
$3,375.00 /1 Kit

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Specifications
Analysis Time
  • Short array 50 min
Assay Sensitivity
  • Standard
DNA Sizing Range
  • 100 - 6000 bp
Fragment Sensitivity Range
  • 0.1 - 10 ng/µL
Guaranteed Shelf Life
  • Minimum 4 months
Kit Type
  • Quantitative
Quantitative Accuracy
  • ± 25 %
Quantitative Precision
  • 15 % CV
Sample Volume
  • 2 µL
Sizing Accuracy
  • ± 5 %
Sizing Precision
  • 2 % CV
Sizing Resolution
  • 100 - 1000 bp: ≤ 5 %
  • 1000 - 6000 bp: ≤ 10 %
Smear Sensitivity Range
  • 5 - 100 ng/µL
Uses Per Kit
  • 1000
Kit Contents
Part Number Product Description Quantity
DNF-240-0500 NGS Fragment Separation Gel, 500mL 1
DNF-302-0008 BF-2000 Blank Solution, 8mL 1
DNF-355-0300 5x 930 dsDNA Inlet Buffer, 300mL 1
DNF-374-0003 NGS Diluent Marker (1-6000 bp), 2.4 mL 10
DNF-399-U100 NGS DNA Ladder, 100 µL 2
DNF-475-0050 5x Capillary Conditioning Solution, 50mL 1
DNF-497-0125 0.25x TE Rinse Buffer, 125mL 1
DNF-600-U030 Intercalating Dye, 30 µL 2
Support
User Manuals

Agilent DNF-473 NGS Fragment Kit (1-6000 bp) Quick Guide for Fragment Analyzer Systems

This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The NGS Fragment kit (1–6000 bp) (p/n DNF-473- 0500 and DNF-473-1000) is used for the quantitative and qualitative analysis of NGS libraries and their intermediates from 100 to 6,000 bp.