GK80030
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Exoglycosidases

Part Number:
GK80030

AdvanceBio Sialidase C, 1 U. Enzyme releases α(2-3,6)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates. Includes 5x reaction buffer B [250 mM sodium phosphate pH 6.0].

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Specifications
Concentration
  • 10 U/mL
Enzyme Formulation
  • 20 mM Tris HCl pH 7.5, containing 25 mM NaCl
Enzyme Source
  • Recombinant from Clostridium perfringens, expressed in E. coli. Formerly known as product code GE24. Sialidases are also known as neuraminidases.
Enzyme Specific Activity
  • ≥40 units/mg
Enzyme Specificity
  • This enzyme releases a2-3, and a2-6 linked N-acetylneuraminic acid from complex carbohydrates. This enzyme will not efficiently cleave NeuAc a2-6 linked to N-acetylgalactosamine (GalNAc) when the GalNAc is labeled with a fluorophore. Sialidase A (GK80040) is recommended for this application.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to catalyze the release of 1 μmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37°C, pH 5.5
Molecular Weight
  • ~41,000 daltons
Unit
  • 1 U
Volume
  • 100 µL
pH Optimum
  • 6
Support
Safety Data Sheets

AdvanceBio Sialidase C, 5x Reaction Buffer B

AdvanceBio Sialidase C, 1 Unit(100 μl), 5x Reaction Buffer B, 1 ml

AdvanceBio Sialidase C, 5x Reaction Buffer B

AdvanceBio Sialidase C, 1 Unit(100 μl), 5x Reaction Buffer B, 1 ml

AdvanceBio Sialidase C, 5x Reaction Buffer B

AdvanceBio Sialidase C, 1 Unit(100 μl), 5x Reaction Buffer B, 1 ml

User Manuals

GK80030 Sialidase C user manual

User manual for GK80030 Sialidase C