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BL21 Competent Cells

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BL21-Gold (DE3) Competent Cells. For non-toxic proteins, encodes T7 RNA polymerase under the control of the lacUV5 promoter. Use with Variflex or Affinity pCAL vectors. Efficiency is ≥1 x 108 cfu/µg of supercoiled DNA.

For Research Use Only. Not for use in diagnostic procedures.
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Cell Strain
  • BL21 (DE3)
Cell Type - Production Method
  • Chemically Competent
Competent Cell Suitable For
  • High expression level with IPTG expression control
Difficult Protein Expression Type
  • Toxic Proteins
Generates Unmethylated DNA
  • No
  • E. coli B F– ompT hsdS(rB– mB–) dcm+ Tetr gal l(DE3) endA The
  • Tetracycline
Transformation Efficiency - Quantitative
  • 1 x 10^8
  • 10 x 0.1 mL