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BL21 Competent Cells

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BL21 Competent Cells. For extremely toxic proteins, use with lambda CE6 under the control of T7 RNA polymerase or use with non-T7 polymerase-based systems

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Cell Strain
  • BL21
Cell Type - Production Method
  • Chemically Competent
Competent Cell Suitable For
  • Tightest control of uninduced expression using lCE6
Difficult Protein Expression Type
  • Toxic Proteins
Generates Unmethylated DNA
  • No
  • E. coli B F– dcm ompT hsdS(rB– mB–) gal
  • Tetracycline
Transformation Efficiency - Quantitative
  • 1 x 10^6
  • 5 x 0.2 mL