GKX-5013
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Exoglycosidases

Part Number:
GKX-5013

β(1-3,4)-Galactosidase (bovine testis) (formerly ProZyme). Enzyme hydrolyzes nonreducing terminal galactose β(1-3) and β(1-4) linkages. Can be used with other β-galactosidases for exoglycosidase sequencing. Includes  5x reaction buffer (500 mM sodium citrate/phosphate pH 4.0).

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List Price:
$336.00

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Specifications
Concentration
  • 5 U/mL
Enzyme Applications
  • The enzyme has applications in the analysis of a wide variety of glycoconjugates. It is particularly useful for ensuring the complete removal of ß(1-3) and ß(1-4)-linked non-reducing terminal galactose residues of oligosaccharides. Gal ß(1-6) GlcNAc is hydrolyzed more slowly, however this linkage is not normally encountered in native complex glycans. This activity towards ß(1-3) and ß(1-4)-linked galactose contrasts with that of our other ß-galactosidases (e.g. from S. pneumoniae, GKX-5014, or Jack bean, GKX-5012) which exhibit a preference for Gal ß(1-4), and cleave the Gal ß(1-3) linkage relatively slowly, if at all. Used in conjunction, these enzymes provide a powerful means to determine linkage positions of non-reducing ß galactose residues.
Enzyme Formulation
  • 20 mM sodium citrate phosphate, 150 mM NaCl (pH 4.0)
Enzyme Source
  • Bovine testis.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to hydrolyze 1 µmole of pNP-ß-D-galactopyranoside per minute at pH 4.0 and 37°C.
Molecular Weight
  • ~68 kD
Unit
  • 0.5 U
Volume
  • 100 µL
pH Optimum
  • 4
Support
Safety Data Sheets

β(1-3,4)-Galactosidase (bovine testis)

β(1-3,4)-Galactosidase (bovine testis), 100 μl, 5x Reaction Buffer, 1 ml

β(1-3,4)-Galactosidase (bovine testis)

β(1-3,4)-Galactosidase (bovine testis), 100 μl, 5x Reaction Buffer, 1 ml

β(1-3,4)-Galactosidase (bovine testis)

β(1-3,4)-Galactosidase (bovine testis), 100 μl, 5x Reaction Buffer, 1 ml

β(1-3,4)-Galactosidase (bovine testis)

β(1-3,4)-Galactosidase (bovine testis), 100 μl, 5x Reaction Buffer, 1 ml