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Endoglycosidases

Part Number:
GKE-5016B-1

AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL (formerly ProZyme). A recombinant form of PNGase F, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Supplied as 5 x 200 mU vials of GKE-5016B. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications).

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List Price:
$1,653.00

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Specifications
Enzyme Source
  • Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specificity
  • Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Unit
  • 1000 mU
Literature
Data Sheets

Agilent AdvanceBio N-Glycanase

Agilent AdvanceBio N-Glycanase for the release of intact N-linked glycans from glycopeptides and glycoproteins and structure-function studies of N-glycosylated glycoproteins.

Support
Safety Data Sheets

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F, EDTA-Free), ≥2.5 U/ml