DNF-474-0500
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Fragment Analyzer DNA/NGS Kits

Part Number:
DNF-474-0500
RUO

HS NGS Fragment Kit (1-6000bp), 500. Fragment Analyzer system kit for the quantitative and qualitative analysis of NGS libraries and intermediates from 100 to 6000 bp at low concentrations. Includes reagents necessary for running 500 samples.

For Research Use Only. Not for use in diagnostic procedures.
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List Price:
$1,686.00 /1 Kit

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Specifications
Assay Sensitivity
  • High
DNA Sizing Range
  • 100-6000 bp
Fragment Sensitivity Range
  • 5–500 pg/µL
Guaranteed Shelf Life
  • Minimum 4 months
Kit Type
  • Quantitative
Quantitative Accuracy
  • ± 25 %
Quantitative Precision
  • 15 % CV
Sample Volume
  • 2 µL
Sizing Accuracy
  • ± 5 %
Sizing Reproducibility
  • 2 % CV
Sizing Resolution
  • 100-2000 bp: ≤ 5 %
  • 2000-5000 bp: ≤ 10 %
Smear Sensitivity Range
  • 50-5000 pg/µL
Uses Per Kit
  • 500
Kit Contents
Part Number Product Description Quantity
DNF-240-0240 NGS Fragment Separation Gel, 240mL 1
DNF-300-0008 BF-25 Blank Solution, 8mL 1
DNF-355-0125 5x 930 dsDNA Inlet Buffer, 125mL 1
DNF-373-0003 HS NGS Diluent Marker (1-6000 bp), 2.4 mL 5
DNF-396-U100 HS NGS DNA Ladder, 100uL 1
DNF-475-0050 5x Capillary Conditioning Solution, 50mL 1
DNF-497-0125 0.25x TE Rinse Buffer, 125mL 1
DNF-600-U030 Intercalating Dye, 30 µL 1
Support
User Manuals

Agilent DNF-474 HS NGS Fragment Kit Quick Guide for Fragment Analyzer Systems

This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The DNF-474 HS NGS Fragment assay is designed for the quantitative and qualitative analysis of NGS libraries and their intermediates from 100 to 6,000 bp.