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Endoglycosidases

Part Number:
WS0278-GP

N-glycanase, for GlykoPrep-plus, 700 μL. Replacement enzyme for GlykoPrep-plus kits, 620 ul fill, ≥10 U/mL, EDTA-free

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List Price:
$4,988.00

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Specifications
Application
  • Refill for GlykoPrep-plus glaycan preparatation kit
Enzyme Source
  • Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specificity
  • Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate and sialic, acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.