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AdvanceBio Gly-X Technology

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AdvanceBio Gly-X N-glycan prep with InstantPC kit, 96-ct. Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantPC dye. After a simple cleanup step, the glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS and other methods. With deglycosylation and labeling carried out in solution, the method is simple, rapid, and suitable for automation. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, that enables a single labeling method to be deployed across different glycan analysis workflows. Sample preparation time: ~1 hour. Includes GX96-100 Gly-X deglycosylation module, GX96-101 Gly-X InstantPC labeling module, GX96-102 Gly-X InstantPC cleanup module.

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  • Flexible, high-throughput format: process 1 to 96 samples
  • 96
Equipment & Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks
  • Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold and pump other than the Millipore model suggested, please contact us for information. Alternative vacuum manifold may be used with a GX200 spacer in place of GX100 (see FAQs)
Kit Configuration
  • Sample Prep Kit
  • InstantPC
Precision (well-to-well, day-to-day)
  • <5% CV
Prep time (approx)
  • ~1 hour
Preservation of Sialylated Species
  • > 95 %
Protein Load Range
  • 1-40 µg
  • > 95 %
  • Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF
  • LC & LC/MS
Kit Contents
Part Number Product Description Quantity
GX96-100 AdvanceBio Gly-X deglycosylation module, 96-ct 1
GX96-101 AdvanceBio Gly-X InstantPC labeling module, 96-ct 1
GX96-102 AdvanceBio Gly-X InstantPC cleanup module, 96-ct 1

High-Throughput CE N-Glycan Analysis for Clone Screening

Rapid N-glycan preparation and screening of biosimilar candidates by LC/FLD/MS and CE.

Streamlined Workflows for N-Glycan Analysis of Biotherapeutics with LC/FLD/MS

Streamlined workflows for N-Glycan analysis of biotherapeutics using Agilent AdvanceBio Gly-X InstantPC and 2-AB Express sample preparation with LC/FLD/MS

User Manuals

Agilent AdvanceBio Gly-X N-Glycan Prep with InstantPC Kit, 96-ct

The Agilent Gly-X N-Glycan Rapid Release and Labeling with InstantPC kit utilizes a novel in-solution enzymatic protein deglycosylation with N-Glycanase followed by fast labeling of released N-glycans with InstantPC dye.

Agilent AdvanceBio Gly-X InstantPC Quick Start Guide

Prepare N-glycan samples in 1 hour using Agilent AdvanceBio Gly-X InstantPC with this simple step by step process.