GX96-402
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AdvanceBio Gly-X Technology

Part Number:
GX96-402

AdvanceBio Gly-X 2-AB Express cleanup module, 96-ct. Module for use with Gly-X N-glycan prep and labeling with 2-AB Express modules. This cleanup plate is used for on-matrix 2-AB labeling as well as cleanup of free dye.

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Specifications
Application
  • Optimized cleanup removes excess free dye, protein, and other interfering compounds.
Count
  • 96
Deglycosylation Efficiency
  • > 99 %
Equipment & Reagents Provided by User
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)
  • DI water
  • Gly-X deglycosylation and 2-AB Express labeling 24-ct [GX24-401AB] or 96-ct [GX96-401AB]
  • ProZyme vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB starter kit, included free of charge with first Gly-X order, please contact us for details)
  • Vacuum manifold (Millipore MSVMHTS00-HTS)
  • Vacuum pump (Millipore WP6211560, 110V. WP6122050, 220V. Welch WOB-L Pump 2522)
Kit Configuration
  • Sample Prep Module
Label
  • 2-AB
Precision (well-to-well, day-to-day)
  • <5% CV
Prep time (approx)
  • 130 min (24 samples)
Preservation of Sialylated Species
  • > 99 %
Protein Load Range
  • 1-40 µg
Recovery
  • > 80 %
Stability
  • At least 1 month at -20°C, 5 days at 4–8°C
Technique
  • LC & LC/MS