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AdvanceBio Gly-X deglycosylation and InstantQ labeling module set, 96-ct (formerly ProZyme). Utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantQ dye. InstantQ dye is specifically designed for glycan separation and detection using the Gly-Q instrument and a Gly-Q InstantQ cartridge. Includes GX96-300 Gly-X deglycosylation module for InstantQ, GX96-301 Gly-X InstantQ labeling module. (for cleanup use GX96-302 Gly-X InstantQ cleanup module).

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/96 Pack
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Specifications
Application
  • Flexible, high-throughput format: process 1 to 96 samples
Count
  • 96
Deglycosylation Efficiency
  • > 99 %
Equipment & Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks, set for 90 °C and 50 °C
  • Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended)
  • Formic acid, MS-grade (Fisher A117-50 recommended)
  • Thermocycler (Corning THERM-1001, 110 V. THERM-1000, 230 V)
  • Vacuum manifold (Millipore MSVMHTS00-HTS)
  • Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220 V. Welch WOB-L pump 2522)
Instrument Compatibility
  • Gly-Q Glycan Analysis System
Kit Configuration
  • Sample Prep Module Set
Label
  • InstantQ
Precision (well-to-well, day-to-day)
  • <5% CV
Prep time (approx)
  • ~1 hour
Preservation of Sialylated Species
  • > 95 %
Protein Load Range
  • 1-40 µg
Recovery
  • > 85 %
Stability
  • Up to 18 hrs on RT autosampler, and -20°C for up to 30 days in water
Technique
  • CE