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AdvanceBio Gly-X Technology

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AdvanceBio Gly-X deglycosylation and InstantAB labeling module set, 96-ct (formerly ProZyme). Utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid instant labeling of released N-glycans with InstantAB dye. With deglycosylation and labeling carried out in solution, the method is simple, rapid, and suitable for automation. After a simple cleanup step, the InstantAB-labeled glycan samples are ready for analysis by UHPLC, LC/MS, MS/MS and other methods. Includes GX96-100 Gly-X deglycosylation module, GX96-501 Gly-X InstantAB labeling module.

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  • Flexible, high-throughput format: process 1 to 96 samples
  • Instant labeling with InstantAB:5-minute N-Glycanase (PNGase F) digestion provides unbiased N-glycan release
  • 96
Equipment and Reagents Provided by User
  • 96-well Thermocycler or two independent heat blocks, set for 90°C and 50°C. (e.g. Corning THERM-1001, 110V. THERM-1000, 230V). Note: Two GlykoPrep heaters, GS150, can be fitted with VWR 13259-260 modular heating blocks.
  • Vacuum pump (Millipore WP6211560, 110 V, WP6122050, 220V, Welch WOB-L Pump 2522). Note: If you have a vacuum manifold other than the Millipore model suggested, contact us for setup instructions
Kit Configuration
  • Sample Prep Module Set
  • InstantAB
Prep time (approx)
  • ~1 hour
Protein Load Range
  • 1-40 µg
  • Samples may be stored at -20°C for at least 6 months or 4°C for up to 5 days.
  • LC
Kit Contents
Part Number Product Description Quantity
GX96-100 AdvanceBio Gly-X deglycosylation module, 96-ct (formerly ProZyme) 1
GX96-501 AdvanceBio Gly-X InstantAB labeling module, 96-ct (formerly ProZyme) 1