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GlykoPrep-plus Technology

Part Number:
GPPNG-PC

GlykoPrep-plus N-glycan prep with InstantPC, 96-ct (formerly ProZyme). Complete kit configured for automation on the Agilent AssayMAP Bravo liquid handling platform. InstantPC dye is a novel instant glycan labeling reagent for MS that provides increased MS and fluorescent sensitivity. InstantPC forms a stable urea linkage with glycosylamines released by rapid N-glycanase (PNGase F) digestion. GlykoPrep N-glycan sample preparation is a cartridge based workflow and utilizes a rapid digestion, instant labeling, and cleanup of excess InstantPC label. Includes GSP-RX digestion module, GSP-PC InstantPC labeling module, GS96-CU cleanup module, AM96-NG GlykoPrep-plus labware set

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List Price:
$7,559.00 /96 Pack

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Specifications
Count
  • 96
Equipment and Reagents Provided by User
  • 100% Acetonitrile (~250 mL, HPLC-grade)
  • Calibrated pipettes and disposable tips (P5/10, P200 and P1000)
  • Formic acid (HPLC-grade)
  • Glass bottle with screw cap (50 mL, 1 ea)
  • Glass graduated cylinder (50 mL, 1 ea)
  • Nitrile gloves
  • Ultrapure, deionized water (~100 mL, Milli-Q or equivalent)
  • Volumetric pipettes (5 mL, 1 ea)
  • Vortexer
Instrument Compatibility
  • AssayMAP Bravo Platform
Kit Configuration
  • Sample Prep Kit
Label
  • InstantPC
Prep time (approx)
  • 3 hours
Technique
  • LC & LC/MS
Kit Contents
Part Number Product Description Quantity
- InstantPC Labeling Module (for Bravo) 1
AM96-NG GlykoPrep-plus labware set (formerly ProZyme) 1
GS96-CU GlykoPrep cleanup module, 96-ct (formerly ProZyme) 1
GSP-RX GlykoPrep-plus digestion module, 96-ct (fomerly ProZyme) 1
Literature
Applications

Analysis of Monoclonal Antibody N-glycans by Fluorescence Detection and Mass Selective Detection Using LC/MSD XT

This app note demonstrates the performance of an Agilent LC/MSD to solve ambiguous peak assignments for the analysis of N-glycosylation of therapeutic proteins

A Comprehensive Approach for Monoclonal Antibody N-linked Glycan Analysis from Sample Preparation to Data Analysis

This application note demonstrates how to increase sample throughput for glycan characterization workflows using the Agilent biopharmaceutical solution.

Support
User Manuals

GPPNG-PC Instruction Manual

User manual for GPPNG-PC Instruction Manual