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GlykoPrep Technology

Part Number:
GP24NG-PC

GlykoPrep N-glycan prep with InstantPC dye, 24-ct complete kit (formerly ProZyme) a novel instant glycan labeling reagent for MS that provides increased MS and fluorescent sensitivity. InstantPC forms a stable urea linkage with glycosylamines released by N-Glycanase (PNGase F) digestion. GlykoPrep N-glycan sample preparation is a cartridge based workflow with rapid digestion, instant labeling, and cleanup of excess InstantPC label. Includes GS24-RX digestion module; GS24-PC InstantPC labeling module; GS24-CU cleanup module.

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List Price:
$2,938.00

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Specifications
Application
  • Optional protein A purification for monoclonal antibodies and Fc proteins (G5524-60010 KIT)
  • Nonselective, rapid release and recovery of intact N-Glycans from up to 24 glycoprotein samples at a time using a microplate centrifuge
  • Nonselective chemistry for stoichiometric labeling of glycans, independent of structure
  • Flexible, format: process 1 to 24 samples per run
  • InstantDye labeling of N-glycans with InstantPC provides high fluorescence and MS signal
  • Labeled glycans are eluted in low volume and ready for analysis without concentrating or drying
Kit Configuration
  • Sample Prep Kit
Count
  • 24
Technique
  • LC & LC/MS
Label
  • InstantPC
Prep time (approx)
  • 3 hours
Equipment and Reagents Provided by User
  • Heater and incubation blocks, capable of 50–100°C, available from Agilent (GS150)
  • AssayMAP Labware (AM200)
  • Centrifuge (capable of 50 - 1000 x g) and deep microplate rotor with clearance of minimum 44 mm height
  • Acetonitrile (ACN, 100%, HPLC-grade)
  • Formic acid (HPLC-grade)
Resources

2-AB Microfuge Method

User manual for 2-AB Microfuge Method

InstantPC Microfuge Method

User manual for Microfuge IPC

GlykoPrep Rapid N-Glycan Preparation with InstantPC™

User manual for GP96NG-PC Rapid N-Glycan Preparation with InstantPC

Immobilization Reagent Set, GlykoPrep Digestion Reagent Set, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, InstantPC Dye Solvent, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, 24-ct, GlykoPrep Digestion Reagent Set, 24-ct, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, 30 mg, InstantPC Dye Solvent, 600 μl, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, GlykoPrep Digestion Reagent Set, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, InstantPC Dye Solvent, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, 24-ct, GlykoPrep Digestion Reagent Set, 24-ct, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, 30 mg, InstantPC Dye Solvent, 600 μl, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, GlykoPrep Digestion Reagent Set, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, InstantPC Dye Solvent, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, 24-ct, GlykoPrep Digestion Reagent Set, 24-ct, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, 30 mg, InstantPC Dye Solvent, 600 μl, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, GlykoPrep Digestion Reagent Set, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, InstantPC Dye Solvent, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, 24-ct, GlykoPrep Digestion Reagent Set, 24-ct, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, 30 mg, InstantPC Dye Solvent, 600 μl, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, GlykoPrep Digestion Reagent Set, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, InstantPC Dye Solvent, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, 24-ct, GlykoPrep Digestion Reagent Set, 24-ct, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, 30 mg, InstantPC Dye Solvent, 600 μl, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, GlykoPrep Digestion Reagent Set, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, InstantPC Dye Solvent, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, 24-ct, GlykoPrep Digestion Reagent Set, 24-ct, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, 30 mg, InstantPC Dye Solvent, 600 μl, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, GlykoPrep Digestion Reagent Set, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, InstantPC Dye Solvent, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, 24-ct, GlykoPrep Digestion Reagent Set, 24-ct, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, 30 mg, InstantPC Dye Solvent, 600 μl, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, GlykoPrep Digestion Reagent Set, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, InstantPC Dye Solvent, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, 24-ct, GlykoPrep Digestion Reagent Set, 24-ct, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, 30 mg, InstantPC Dye Solvent, 600 μl, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, GlykoPrep Digestion Reagent Set, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, InstantPC Dye Solvent, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, 24-ct, GlykoPrep Digestion Reagent Set, 24-ct, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, 30 mg, InstantPC Dye Solvent, 600 μl, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, GlykoPrep Digestion Reagent Set, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, InstantPC Dye Solvent, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, 24-ct, GlykoPrep Digestion Reagent Set, 24-ct, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, 30 mg, InstantPC Dye Solvent, 600 μl, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, GlykoPrep Digestion Reagent Set, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, InstantPC Dye Solvent, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, 24-ct, GlykoPrep Digestion Reagent Set, 24-ct, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, 30 mg, InstantPC Dye Solvent, 600 μl, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, GlykoPrep Digestion Reagent Set, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, InstantPC Dye Solvent, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, 24-ct, GlykoPrep Digestion Reagent Set, 24-ct, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, 30 mg, InstantPC Dye Solvent, 600 μl, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, GlykoPrep Digestion Reagent Set, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, InstantPC Dye Solvent, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, 24-ct, GlykoPrep Digestion Reagent Set, 24-ct, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, 30 mg, InstantPC Dye Solvent, 600 μl, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, GlykoPrep Digestion Reagent Set, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, InstantPC Dye Solvent, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, 24-ct, GlykoPrep Digestion Reagent Set, 24-ct, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, 30 mg, InstantPC Dye Solvent, 600 μl, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, GlykoPrep Digestion Reagent Set, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, InstantPC Dye Solvent, GlykoPrep Cleanup (CU) Cartridges

Immobilization Reagent Set, 24-ct, GlykoPrep Digestion Reagent Set, 24-ct, GlykoPrep Digestion (RX) Cartridges, InstantPC Dye, 30 mg, InstantPC Dye Solvent, 600 μl, GlykoPrep Cleanup (CU) Cartridges