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GlykoPrep Technology

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GlykoPrep rapid N-glycan prep with 2-AB, 24-ct (formerly ProZyme). Complete kit is a cartridge based workflow with rapid digestion, labeling, and cleanup of excess 2-AB label. Includes GS24-RX digestion module, GS24-AB Reductive-Amination 2-AB labeling module, GS24-CU cleanup module.

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Equipment and Reagents Provided by User
  • Heater and incubation blocks, capable of 50–100°C, available from Agilent (GS150)
  • AssayMAP Labware (AM200)
  • Centrifuge (capable of 50 - 1000 x g) and deep microplate rotor with clearance of minimum 44 mm height
  • Ultrapure, deionized water
  • Acetonitrile (ACN, 100%, HPLC-grade)
  • Formic acid (HPLC-grade)
  • 2-AB
Prep time (approx)
  • 4 hours
  • Nonselective, rapid release and recovery of intact N-glycans
  • Flexible format
  • Rapid-reductive-amination labeling with 2-AB
  • Purified, fluorescently labeled N-glycans are produced in water and ready for analysis without concentrating or drying
  • Compatible with microplate liquid handling on a broad range of automation platforms
  • 24
  • LC & LC/MS
Kit Configuration
  • Sample Prep Kit
Kit Contents
Part Number Product Description Quantity
GS24-CU GlykoPrep cleanup module, 24-ct (formerly ProZyme) 1
GS24-AB GlykoPrep 2-AB labeling module, 24-ct (formerly ProZyme) 1
GS24-RX GlykoPrep digestion module, 24-ct (formerly ProZyme) 1

Development of a Rapid 2-AB Sample Preparation Workflow for N-Glycans

The characterization of N-glycans is essential to the development of biotherapeutics.