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Endoglycosidases

Part Number:
GKE-5020D-10

AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), ≥10 U/mL. Enzyme (a recombinant form of PNGase F, EDTA free) releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. The same formulation provided in GlykoPrep, ≥ 10 U/mL and EDTA-free. Supplied as 10 vials of GKE-5020D (10 x 1 U, ≥10 U/ml). GKE-5020D pack size does not ship with reaction buffers, although reaction buffers are available on request.

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Specifications
Concentration
  • ≥10 U/mL
Enzyme Applications
  • To obtain efficient deglycosylation of glycoprotein substrates under non-denaturing conditions, it is necessary to use a higher starting concentration of enzyme. N-Glycanase PLUS and ULTRA (EDTA-Free) are supplied at = 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller reaction volumes and shorter reaction times to be explored.
Enzyme Formulation
  • 20 mM Tris HCl pH 7.5, 50 mM NaCl
Enzyme Source
  • Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity
  • ≥10 units/mg
Enzyme Specificity
  • Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight
  • ~35,000 Daltons
Unit
  • 10 U
Volume
  • 100 µL
pH Optimum
  • 8.6
pH Range
  • 7.5-9.5
Literature
Data Sheets

Agilent AdvanceBio N-Glycanase-ULTRA

Agilent AdvanceBio N-Glycanase ULTRA for the removal of N-glycans from glycoproteins and glycopeptides.

  • Data Sheets
  • English
  • 30-Jul-2019
  • 571.71 Kb
  • PDF
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Support
Safety Data Sheets

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

  • Safety Data Sheets
  • English
  • 12-May-2019
  • 198.08 Kb
  • PDF

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

  • Safety Data Sheets
  • English
  • 12-May-2019
  • 69.05 Kb
  • PDF

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

  • Safety Data Sheets
  • English
  • 12-May-2019
  • 170.32 Kb
  • PDF

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

AdvanceBio N-Glycanase-ULTRA (PNGase F, EDTA-Free), ≥10 U/mL

  • Safety Data Sheets
  • English
  • 12-May-2019
  • 166.62 Kb
  • PDF

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