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Endoglycosidases

Part Number:
GKE-5006B-1

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/mL (formerly ProZyme). A recombinant form of PNGase F, releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Supplied as 5 x 200 mU vials of GKE-5006B. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications). Supplied at a concentration of ≥ 2.5 U/mL. For a more concentrated formulation of ≥ 10 U/mL, which may be useful for native digestions, see GKE-5010B or GKE-5020B (EDTA-free).

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Specifications
Concentration
  • ≥2.5 U/mL
Enzyme Applications
  • Preparation of deglycosylated proteins for molecular weight estimation or crystallography studies
  • Release of intact N-linked glycans from glycopeptides and glycoproteins
  • Structure-function studies of N-glycosylated glycoproteins
Enzyme Formulation
  • 20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA (pH 7.5)
Enzyme Source
  • Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. The enzyme is also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity
  • ≥10 units/mg
Enzyme Specificity
  • Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless α(1-3) core fucosylated, as in plant glycans and some insect glycans. Asparagine must be peptide-bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. This product is Endo F free.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight
  • ~35,000 daltons
Unit
  • 1000 mU
Volume
  • 80 µL
pH Optimum
  • 8.6
pH Range
  • 7.5-9.5
Literature
Data Sheets

Agilent AdvanceBio N-Glycanase

Agilent AdvanceBio N-Glycanase for the release of intact N-linked glycans for glycopeptides and glycoproteins and structure-function study of N-glycosylated glycoproteins.

  • Data Sheets
  • English
  • 30-Jul-2019
  • 588.50 Kb
  • PDF
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Support
Safety Data Sheets

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/ml

  • Safety Data Sheets
  • English
  • 30-Apr-2019
  • 158.76 Kb
  • PDF

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/ml

  • Safety Data Sheets
  • English
  • 30-Apr-2019
  • 160.92 Kb
  • PDF

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/ml

  • Safety Data Sheets
  • English
  • 30-Apr-2019
  • 164.37 Kb
  • PDF

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/ml

AdvanceBio N-Glycanase (PNGase F), ≥2.5 U/ml

  • Safety Data Sheets
  • English
  • 30-Apr-2019
  • 184.01 Kb
  • PDF

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