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Exoglycosidases

Part Number:
GK80043

AdvanceBio Sialidase A, Sampler Kit. Contains lower MW sialidase A isoforms for challenging sialidase digests. This sampler kit contains 1 vial each of 3 isoforms (88, 66 and 51 kDa) of the enzyme Sialidase A. Lower MW sialidases may help desialylate glycans if steric hindrance is an issue with regular sialidase.

Each enzyme vial contains 200 mU in 40 µl. Also included is one vial 5x reaction buffer (1 mL). These products are available for purchase separately in 1-Unit pack sizes under the individual product codes GK80040 (88 kDa), GK80046 (66 kDa) and GK80045 (51 kDa).

Sialidases are also known as neuraminidases.

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List Price:
$353.00

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Specifications
Concentration
  • 5 U/mL
Enzyme Source
  • Recombinant gene from Arthrobacter ureafaciens expressed in E. coli. Sialidases are also known as neuraminidases.
Enzyme Specificity
  • The enzyme releases a(2,3)-, a(2,6)-, a(2,8)-, and a(2,9)-linked N-acetylneuraminic acid from complex carbohydrates. The initial rate of hydrolysis of a(2,6) linkages is reported to be approximately twice that of a(2,3)-linked sialic acid however, in practice, this kinetic selectivity is of little consequence during extended incubations. Effective digestion of glycolipid substrates is facilitated by addition of a detergent, such as sodium taurodeoxycholate to the incubation. Sialidase A is capable of releasing N-glycolylneuraminic acid (Neu5Gc, NGNA) in addition to N-acetylneuraminic acid (Neu5Ac, NANA) [1], although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac. The enzyme can also be used to remove sialic acid from gangliosides [3], glycosphingolipids (ceramide and oligosaccharide) with sialic acid. May be used for exoglycosidase sequencing with Sialidase S (GK80021), which is specific for a(2,3)-linked sialic acids. Sialidases are also known as neuraminidase.
Enzyme Unit Definition
  • One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37° C, pH 5.5
pH Optimum
  • 6
Support
Safety Data Sheets

AdvanceBio Sialidase A, AdvanceBio Sialidase A 51, AdvanceBio Sialidase A 66, 5x Reaction Buffer B

AdvanceBio Sialidase A, 1 Unit (200 μl), AdvanceBio Sialidase A 51, 1 Unit (200 μl), AdvanceBio Sialidase A 66, 1 Unit (200 μl), 5x Reaction Buffer B, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 471.03 Kb
  • PDF

AdvanceBio Sialidase A, AdvanceBio Sialidase A 51, AdvanceBio Sialidase A 66, 5x Reaction Buffer B

AdvanceBio Sialidase A, 1 Unit (200 μl), AdvanceBio Sialidase A 51, 1 Unit (200 μl), AdvanceBio Sialidase A 66, 1 Unit (200 μl), 5x Reaction Buffer B, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 448.76 Kb
  • PDF

AdvanceBio Sialidase A, AdvanceBio Sialidase A 51, AdvanceBio Sialidase A 66, 5x Reaction Buffer B

AdvanceBio Sialidase A, 1 Unit (200 μl), AdvanceBio Sialidase A 51, 1 Unit (200 μl), AdvanceBio Sialidase A 66, 1 Unit (200 μl), 5x Reaction Buffer B, 1 ml

  • Safety Data Sheets
  • English
  • 15-Jul-2019
  • 452.37 Kb
  • PDF

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